A point mutation of integrin β2 subunit blocks binding of α5β1 to fibronectin and invasin but not recruitment to adhesion plaques

Yoshikazu Takada; Jari Ylänne; David Mandelman; Wilma Puzon; Mark H. Ginsberg

A point mutation of integrin β₂ subunit blocks binding of α₅β₁ to fibronectin and invasin but not recruitment to adhesion plaques

Yoshikazu Takada, Jari Ylänne, David Mandelman, Wilma Puzon, Mark H. Ginsberg

Research output: Contribution to journal › Article

Abstract

A point mutation in a highly conserved region of the β₁ subunit, Asp¹³⁰ to Ala (D130A) substitution, abrogates the Arg-Gly-Asp (RGD)-dependent binding of α₅β₁ to fibronectin (FN) without disrupting gross structure or heterodimer assembly. The D130A mutation also interferes with binding to invasin, a ligand that lacks RGD sequence. In spite of the lack of detectable FN binding by α₅β₁(D130A), it was recruited to adhesion plaques formed on FN by endogenous hamster receptors. Thus, intact ligand binding function is not required for recruitment of α₅β₁ to adhesion plaques. Overexpression of β₁(D130A) partially interfered with endogenous α₅β₁ function, thus defining a dominant negative β₁ integrin mutation.

Original language	English (US)
Pages (from-to)	913-921
Number of pages	9
Journal	Journal of Cell Biology
Volume	119
Issue number	4
State	Published - Nov 1992
Externally published	Yes

Fingerprint

Focal Adhesions

Fibronectins

Point Mutation

Integrins

Ligands

Mutation

Cricetinae

ASJC Scopus subject areas

Cell Biology

Cite this

Takada, Y., Ylänne, J., Mandelman, D., Puzon, W., & Ginsberg, M. H. (1992). A point mutation of integrin β₂ subunit blocks binding of α₅β₁ to fibronectin and invasin but not recruitment to adhesion plaques. Journal of Cell Biology, 119(4), 913-921.

A point mutation of integrin β₂ subunit blocks binding of α₅β₁ to fibronectin and invasin but not recruitment to adhesion plaques. / Takada, Yoshikazu; Ylänne, Jari; Mandelman, David; Puzon, Wilma; Ginsberg, Mark H.

In: Journal of Cell Biology, Vol. 119, No. 4, 11.1992, p. 913-921.

Research output: Contribution to journal › Article

Takada, Y, Ylänne, J, Mandelman, D, Puzon, W & Ginsberg, MH 1992, 'A point mutation of integrin β₂ subunit blocks binding of α₅β₁ to fibronectin and invasin but not recruitment to adhesion plaques', Journal of Cell Biology, vol. 119, no. 4, pp. 913-921.

Takada Y, Ylänne J, Mandelman D, Puzon W, Ginsberg MH. A point mutation of integrin β₂ subunit blocks binding of α₅β₁ to fibronectin and invasin but not recruitment to adhesion plaques. Journal of Cell Biology. 1992 Nov;119(4):913-921.

Takada, Yoshikazu ; Ylänne, Jari ; Mandelman, David ; Puzon, Wilma ; Ginsberg, Mark H. / A point mutation of integrin β₂ subunit blocks binding of α₅β₁ to fibronectin and invasin but not recruitment to adhesion plaques. In: Journal of Cell Biology. 1992 ; Vol. 119, No. 4. pp. 913-921.

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