A Plug-and-Play, Drug-on-Pillar Platform for Combination Drug Screening Implemented by Microfluidic Adaptive Printing

Jiannan Li, Wen Tan, Wenwu Xiao, Randy Carney, Yongfan Men, Yuanpei Li, Gerald Quon, Yousif Ajena, Kit Lam, Tingrui Pan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Traditional high-throughput drug combination screening requires automatic pipetting of drugs into high-density microtiter plates. Here, a drug-on-pillar platform is proposed for efficient combination drug screening. Using the proposed approach, combination drug screening can be carried out in a plug-and-play manner, allowing for high-throughput screening of large permutations of drug combinations at various concentrations, such that drug dispensing and cell-based screening can be temporally separated and therefore can potentially be performed at distant laboratories. The dispensing is implemented using our recently developed microfluidic pneumatic printing platform, which features a low-cost disposable cartridge that minimizes cross contamination. Moreover, our previously developed drug nanoformulation method with amphiphilic telodendrimers has been utilized to maintain drug stability in a dry form, allowing for convenient drug storage, shipping, and subsequent rehydration. Combining the features described above, we have implemented a 1260-spot drug combination array to study the effect of paired drugs against MDA-MB-231 triple negative human breast cancer cells. This study supports the feasibility of the drug-on-pillar platform for combination drug screening and has provided valuable insight into drug combination efficacy against breast cancer.

Original languageEnglish (US)
JournalAnalytical Chemistry
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Drug Combinations
Microfluidics
Printing
Screening
Pharmaceutical Preparations
Throughput
Freight transportation
Pneumatics
Contamination
Cells
Costs

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

A Plug-and-Play, Drug-on-Pillar Platform for Combination Drug Screening Implemented by Microfluidic Adaptive Printing. / Li, Jiannan; Tan, Wen; Xiao, Wenwu; Carney, Randy; Men, Yongfan; Li, Yuanpei; Quon, Gerald; Ajena, Yousif; Lam, Kit; Pan, Tingrui.

In: Analytical Chemistry, 01.01.2018.

Research output: Contribution to journalArticle

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