A physical map of the human Y chromosome

C. A. Tilford, T. Kuroda-Kawaguchi, H. Skaletsky, S. Rozen, L. G. Brown, M. Rosenberg, John Douglas Mcpherson, K. Wylie, M. Sekhon, T. A. Kucaba, R. H. Waterston, D. C. Page

Research output: Contribution to journalArticle

164 Citations (Scopus)

Abstract

The non-recombining region of the human Y chromosome (NRY), which comprises 95% of the chromosome, does not undergo sexual recombination and is present only in males. An understanding of its biological functions has begun to emerge from DNA studies of individuals with partial Y chromosomes, coupled with molecular characterization of genes implicated in gonadal sex reversal, Turner syndrome, graft rejection and spermatogenic failure. But mapping strategies applied successfully elsewhere in the genome have faltered in the NRY, where there is no meiotic recombination map and intrachromosomal repetitive sequences are abundant. Here we report a high-resolution physical map of the euchromatic, centromeric and heterochromatic regions of the NRY and its construction by unusual methods, including genomic clone subtraction and dissection of sequence family variants. Of the map's 758 DNA markers, 136 have multiple locations in the NRY, reflecting its unusually repetitive sequence composition. The markers anchor 1,038 bacterial artificial chromosome clones, 199 of which form a tiling path for sequencing.

Original languageEnglish (US)
Pages (from-to)943-945
Number of pages3
JournalNature
Volume409
Issue number6822
DOIs
StatePublished - Feb 15 2001
Externally publishedYes

Fingerprint

Chromosomes, Human, Y
Nucleic Acid Repetitive Sequences
Genetic Recombination
Clone Cells
Bacterial Artificial Chromosomes
Turner Syndrome
Y Chromosome
Graft Rejection
Genetic Markers
Dissection
Chromosomes
Genome
DNA
Genes

ASJC Scopus subject areas

  • Medicine(all)
  • General

Cite this

Tilford, C. A., Kuroda-Kawaguchi, T., Skaletsky, H., Rozen, S., Brown, L. G., Rosenberg, M., ... Page, D. C. (2001). A physical map of the human Y chromosome. Nature, 409(6822), 943-945. https://doi.org/10.1038/35057170

A physical map of the human Y chromosome. / Tilford, C. A.; Kuroda-Kawaguchi, T.; Skaletsky, H.; Rozen, S.; Brown, L. G.; Rosenberg, M.; Mcpherson, John Douglas; Wylie, K.; Sekhon, M.; Kucaba, T. A.; Waterston, R. H.; Page, D. C.

In: Nature, Vol. 409, No. 6822, 15.02.2001, p. 943-945.

Research output: Contribution to journalArticle

Tilford, CA, Kuroda-Kawaguchi, T, Skaletsky, H, Rozen, S, Brown, LG, Rosenberg, M, Mcpherson, JD, Wylie, K, Sekhon, M, Kucaba, TA, Waterston, RH & Page, DC 2001, 'A physical map of the human Y chromosome', Nature, vol. 409, no. 6822, pp. 943-945. https://doi.org/10.1038/35057170
Tilford CA, Kuroda-Kawaguchi T, Skaletsky H, Rozen S, Brown LG, Rosenberg M et al. A physical map of the human Y chromosome. Nature. 2001 Feb 15;409(6822):943-945. https://doi.org/10.1038/35057170
Tilford, C. A. ; Kuroda-Kawaguchi, T. ; Skaletsky, H. ; Rozen, S. ; Brown, L. G. ; Rosenberg, M. ; Mcpherson, John Douglas ; Wylie, K. ; Sekhon, M. ; Kucaba, T. A. ; Waterston, R. H. ; Page, D. C. / A physical map of the human Y chromosome. In: Nature. 2001 ; Vol. 409, No. 6822. pp. 943-945.
@article{9ee863f03f8448d4ba0b37b9d9bc6334,
title = "A physical map of the human Y chromosome",
abstract = "The non-recombining region of the human Y chromosome (NRY), which comprises 95{\%} of the chromosome, does not undergo sexual recombination and is present only in males. An understanding of its biological functions has begun to emerge from DNA studies of individuals with partial Y chromosomes, coupled with molecular characterization of genes implicated in gonadal sex reversal, Turner syndrome, graft rejection and spermatogenic failure. But mapping strategies applied successfully elsewhere in the genome have faltered in the NRY, where there is no meiotic recombination map and intrachromosomal repetitive sequences are abundant. Here we report a high-resolution physical map of the euchromatic, centromeric and heterochromatic regions of the NRY and its construction by unusual methods, including genomic clone subtraction and dissection of sequence family variants. Of the map's 758 DNA markers, 136 have multiple locations in the NRY, reflecting its unusually repetitive sequence composition. The markers anchor 1,038 bacterial artificial chromosome clones, 199 of which form a tiling path for sequencing.",
author = "Tilford, {C. A.} and T. Kuroda-Kawaguchi and H. Skaletsky and S. Rozen and Brown, {L. G.} and M. Rosenberg and Mcpherson, {John Douglas} and K. Wylie and M. Sekhon and Kucaba, {T. A.} and Waterston, {R. H.} and Page, {D. C.}",
year = "2001",
month = "2",
day = "15",
doi = "10.1038/35057170",
language = "English (US)",
volume = "409",
pages = "943--945",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "6822",

}

TY - JOUR

T1 - A physical map of the human Y chromosome

AU - Tilford, C. A.

AU - Kuroda-Kawaguchi, T.

AU - Skaletsky, H.

AU - Rozen, S.

AU - Brown, L. G.

AU - Rosenberg, M.

AU - Mcpherson, John Douglas

AU - Wylie, K.

AU - Sekhon, M.

AU - Kucaba, T. A.

AU - Waterston, R. H.

AU - Page, D. C.

PY - 2001/2/15

Y1 - 2001/2/15

N2 - The non-recombining region of the human Y chromosome (NRY), which comprises 95% of the chromosome, does not undergo sexual recombination and is present only in males. An understanding of its biological functions has begun to emerge from DNA studies of individuals with partial Y chromosomes, coupled with molecular characterization of genes implicated in gonadal sex reversal, Turner syndrome, graft rejection and spermatogenic failure. But mapping strategies applied successfully elsewhere in the genome have faltered in the NRY, where there is no meiotic recombination map and intrachromosomal repetitive sequences are abundant. Here we report a high-resolution physical map of the euchromatic, centromeric and heterochromatic regions of the NRY and its construction by unusual methods, including genomic clone subtraction and dissection of sequence family variants. Of the map's 758 DNA markers, 136 have multiple locations in the NRY, reflecting its unusually repetitive sequence composition. The markers anchor 1,038 bacterial artificial chromosome clones, 199 of which form a tiling path for sequencing.

AB - The non-recombining region of the human Y chromosome (NRY), which comprises 95% of the chromosome, does not undergo sexual recombination and is present only in males. An understanding of its biological functions has begun to emerge from DNA studies of individuals with partial Y chromosomes, coupled with molecular characterization of genes implicated in gonadal sex reversal, Turner syndrome, graft rejection and spermatogenic failure. But mapping strategies applied successfully elsewhere in the genome have faltered in the NRY, where there is no meiotic recombination map and intrachromosomal repetitive sequences are abundant. Here we report a high-resolution physical map of the euchromatic, centromeric and heterochromatic regions of the NRY and its construction by unusual methods, including genomic clone subtraction and dissection of sequence family variants. Of the map's 758 DNA markers, 136 have multiple locations in the NRY, reflecting its unusually repetitive sequence composition. The markers anchor 1,038 bacterial artificial chromosome clones, 199 of which form a tiling path for sequencing.

UR - http://www.scopus.com/inward/record.url?scp=0035865360&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035865360&partnerID=8YFLogxK

U2 - 10.1038/35057170

DO - 10.1038/35057170

M3 - Article

C2 - 11237016

AN - SCOPUS:0035865360

VL - 409

SP - 943

EP - 945

JO - Nature

JF - Nature

SN - 0028-0836

IS - 6822

ER -