A pH-sensitive function and phenotype: Evidence that EutH facilitates diffusion of uncharged ethanolamine in Salmonella enterica

Joseph T. Penrod, Christopher C. Mace, John R. Roth

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The eutH gene is part of an operon that allows Salmonella enterica to use ethanolamine as a sole source of nitrogen, carbon, and energy. Although the sequence of EutH suggests a role in transport, eutH mutants use ethanolamine normally under standard conditions (pH 7.0). These mutants fail to use ethanolamine at a low pH. Evidence is presented that protonated ethanolamine (Eth0) does not enter cells, while uncharged ethanolamine (Eth 0) diffuses freely across the membrane. The external concentration of Eth0 varies with the pH (pK = 9.5). At pH 7.0, the standard ethanolamine concentration (41 mM) provides enough Eth0 for an influx rate that can support growth with or without EutH. When a lowered pH and/or ethanolamine concentration reduced the Eth0 concentration below 25 μM, EutH was needed to Facilitate diffusion. EutH+ cells grew normally at Eth0 concentrations above 3 μM, close to the Km (9 μM) of the first degradative enzyme, ethanolamine ammonia lyase. It is suggested that EutH facilitates diffusion of Eth0. As predicted for a transporter, EutH contributed to the toxicity of ethanolamine seen under some conditions; furthermore, fusion of EutH to fluorescent Yfp protein provided evidence that EutH is a membrane protein.

Original languageEnglish (US)
Pages (from-to)6885-6890
Number of pages6
JournalJournal of Bacteriology
Volume186
Issue number20
DOIs
StatePublished - Oct 2004

Fingerprint

Ethanolamine
Salmonella enterica
Phenotype
Ethanolamine Ammonia-Lyase
Operon
Membrane Proteins
Nitrogen
Carbon
Membranes
Enzymes
Growth

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

A pH-sensitive function and phenotype : Evidence that EutH facilitates diffusion of uncharged ethanolamine in Salmonella enterica. / Penrod, Joseph T.; Mace, Christopher C.; Roth, John R.

In: Journal of Bacteriology, Vol. 186, No. 20, 10.2004, p. 6885-6890.

Research output: Contribution to journalArticle

Penrod, Joseph T. ; Mace, Christopher C. ; Roth, John R. / A pH-sensitive function and phenotype : Evidence that EutH facilitates diffusion of uncharged ethanolamine in Salmonella enterica. In: Journal of Bacteriology. 2004 ; Vol. 186, No. 20. pp. 6885-6890.
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