A persistent hindlimb ischemia model in the rabbit

Lee Li-Qun Pu, Steven Jackson, Kevin J. Lachapelle, Zaid Arekat, Alan M. Graham, Robert Lisbona, Roland Brassard, Stirling Carpenter, James F. Symes

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

The study of new approaches for the treatment of limb-threatening ischemia has been hampered by the lack of a suitable animal model of persistent limb ischemia. We describe the development and evaluation of an animal model of persistent hindlimb ischemia, in which ischemia WNS induced in the left hindlimb of 28 rabbits by ligation of the distal external iliac artery arid excision of the common and superficial femoral arteries. The severity of the ischemia and its relief in each animal were evaluated every 10 days postoperatively until day 40 (all animals) or day 90 (five animals). Nine minds developed superficial tissue necrosis in the foot, but no deaths were attributable to the ischemia-inducing procedure. Angiography demonstrated minimal collateralization and sluggish filling of distal vessels up to postoperative day 90. This was accompanied by a decrease at rest in the raw blood flow ratio (p < 0.005 vs day 0), an increase in lactic acid in the femoral venous blood (left vs right side, p < 0.002) up to postoperative day 40, and a decrease in the calf blood pressure ratio (p < 0.0001 vs day 0) up to day 90. Histologic study of the gastrocnemius muscle demonstrated evidence of atrophy und fibrosis in the left hindlimb. This model can be used to evaluate direct and indirect approaches to the treatment of chronic limb ischemia.

Original languageEnglish (US)
Pages (from-to)49-60
Number of pages12
JournalJournal of Investigative Surgery
Volume7
Issue number1
DOIs
StatePublished - 1994
Externally publishedYes

Fingerprint

Hindlimb
Ischemia
Rabbits
Extremities
Animal Models
Iliac Artery
Femoral Artery
Thigh
Atrophy
Ligation
Foot
Lactic Acid
Angiography
Skeletal Muscle
Fibrosis
Necrosis
Blood Pressure

Keywords

  • Animal model
  • Ischemia
  • Lower extremity
  • Rabbit

ASJC Scopus subject areas

  • Surgery

Cite this

Pu, L. L-Q., Jackson, S., Lachapelle, K. J., Arekat, Z., Graham, A. M., Lisbona, R., ... Symes, J. F. (1994). A persistent hindlimb ischemia model in the rabbit. Journal of Investigative Surgery, 7(1), 49-60. https://doi.org/10.3109/08941939409018282

A persistent hindlimb ischemia model in the rabbit. / Pu, Lee Li-Qun; Jackson, Steven; Lachapelle, Kevin J.; Arekat, Zaid; Graham, Alan M.; Lisbona, Robert; Brassard, Roland; Carpenter, Stirling; Symes, James F.

In: Journal of Investigative Surgery, Vol. 7, No. 1, 1994, p. 49-60.

Research output: Contribution to journalArticle

Pu, LL-Q, Jackson, S, Lachapelle, KJ, Arekat, Z, Graham, AM, Lisbona, R, Brassard, R, Carpenter, S & Symes, JF 1994, 'A persistent hindlimb ischemia model in the rabbit', Journal of Investigative Surgery, vol. 7, no. 1, pp. 49-60. https://doi.org/10.3109/08941939409018282
Pu LL-Q, Jackson S, Lachapelle KJ, Arekat Z, Graham AM, Lisbona R et al. A persistent hindlimb ischemia model in the rabbit. Journal of Investigative Surgery. 1994;7(1):49-60. https://doi.org/10.3109/08941939409018282
Pu, Lee Li-Qun ; Jackson, Steven ; Lachapelle, Kevin J. ; Arekat, Zaid ; Graham, Alan M. ; Lisbona, Robert ; Brassard, Roland ; Carpenter, Stirling ; Symes, James F. / A persistent hindlimb ischemia model in the rabbit. In: Journal of Investigative Surgery. 1994 ; Vol. 7, No. 1. pp. 49-60.
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