A partition assay for epoxide hydrases acting on insect juvenile hormone and an epoxide-containing juvenoid

Susanne M. Mumby; Bruce D. Hammock

doi:10.1016/0003-2697(79)90619-5

A partition assay for epoxide hydrases acting on insect juvenile hormone and an epoxide-containing juvenoid

Susanne M. Mumby, Bruce D. Hammock

Entomology

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

An assay for epoxide hydrase activity is described based on the difference in solubility between the epoxide substrate and its corresponding diol. The assay conditions for insect juvenile hormone I and an epoxide-containing juvenoid have been elucidated. In each case, the epoxide partitions into an isooctane phase while the diol partitions largely into an aqueous methanol phase. The assay is faster than chromatographic assays while showing similar or superior precision.

Original language	English (US)
Pages (from-to)	16-21
Number of pages	6
Journal	Analytical Biochemistry
Volume	92
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(79)90619-5
State	Published - Jan 1 1979

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

Access to Document

10.1016/0003-2697(79)90619-5

Cite this

@article{20cce7355ea84c8c8cfe750d525858ea,

title = "A partition assay for epoxide hydrases acting on insect juvenile hormone and an epoxide-containing juvenoid",

abstract = "An assay for epoxide hydrase activity is described based on the difference in solubility between the epoxide substrate and its corresponding diol. The assay conditions for insect juvenile hormone I and an epoxide-containing juvenoid have been elucidated. In each case, the epoxide partitions into an isooctane phase while the diol partitions largely into an aqueous methanol phase. The assay is faster than chromatographic assays while showing similar or superior precision.",

author = "Mumby, {Susanne M.} and Hammock, {Bruce D.}",

year = "1979",

month = jan,

day = "1",

doi = "10.1016/0003-2697(79)90619-5",

language = "English (US)",

volume = "92",

pages = "16--21",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Academic Press Inc.",

number = "1",

}

TY - JOUR

T1 - A partition assay for epoxide hydrases acting on insect juvenile hormone and an epoxide-containing juvenoid

AU - Mumby, Susanne M.

AU - Hammock, Bruce D.

PY - 1979/1/1

Y1 - 1979/1/1

N2 - An assay for epoxide hydrase activity is described based on the difference in solubility between the epoxide substrate and its corresponding diol. The assay conditions for insect juvenile hormone I and an epoxide-containing juvenoid have been elucidated. In each case, the epoxide partitions into an isooctane phase while the diol partitions largely into an aqueous methanol phase. The assay is faster than chromatographic assays while showing similar or superior precision.

AB - An assay for epoxide hydrase activity is described based on the difference in solubility between the epoxide substrate and its corresponding diol. The assay conditions for insect juvenile hormone I and an epoxide-containing juvenoid have been elucidated. In each case, the epoxide partitions into an isooctane phase while the diol partitions largely into an aqueous methanol phase. The assay is faster than chromatographic assays while showing similar or superior precision.

UR - http://www.scopus.com/inward/record.url?scp=0018347287&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018347287&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(79)90619-5

DO - 10.1016/0003-2697(79)90619-5

M3 - Article

C2 - 426275

AN - SCOPUS:0018347287

VL - 92

SP - 16

EP - 21

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 1

ER -

A partition assay for epoxide hydrases acting on insect juvenile hormone and an epoxide-containing juvenoid

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this