A partition assay for epoxide hydrases acting on insect juvenile hormone and an epoxide-containing juvenoid

Susanne M. Mumby, Bruce D. Hammock

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

An assay for epoxide hydrase activity is described based on the difference in solubility between the epoxide substrate and its corresponding diol. The assay conditions for insect juvenile hormone I and an epoxide-containing juvenoid have been elucidated. In each case, the epoxide partitions into an isooctane phase while the diol partitions largely into an aqueous methanol phase. The assay is faster than chromatographic assays while showing similar or superior precision.

Original languageEnglish (US)
Pages (from-to)16-21
Number of pages6
JournalAnalytical Biochemistry
Volume92
Issue number1
DOIs
StatePublished - Jan 1 1979

Fingerprint

Insect Hormones
Epoxide Hydrolases
Juvenile Hormones
Epoxy Compounds
Assays
Solubility
Methanol
Substrates

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

A partition assay for epoxide hydrases acting on insect juvenile hormone and an epoxide-containing juvenoid. / Mumby, Susanne M.; Hammock, Bruce D.

In: Analytical Biochemistry, Vol. 92, No. 1, 01.01.1979, p. 16-21.

Research output: Contribution to journalArticle

@article{20cce7355ea84c8c8cfe750d525858ea,
title = "A partition assay for epoxide hydrases acting on insect juvenile hormone and an epoxide-containing juvenoid",
abstract = "An assay for epoxide hydrase activity is described based on the difference in solubility between the epoxide substrate and its corresponding diol. The assay conditions for insect juvenile hormone I and an epoxide-containing juvenoid have been elucidated. In each case, the epoxide partitions into an isooctane phase while the diol partitions largely into an aqueous methanol phase. The assay is faster than chromatographic assays while showing similar or superior precision.",
author = "Mumby, {Susanne M.} and Hammock, {Bruce D.}",
year = "1979",
month = "1",
day = "1",
doi = "10.1016/0003-2697(79)90619-5",
language = "English (US)",
volume = "92",
pages = "16--21",
journal = "Analytical Biochemistry",
issn = "0003-2697",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - A partition assay for epoxide hydrases acting on insect juvenile hormone and an epoxide-containing juvenoid

AU - Mumby, Susanne M.

AU - Hammock, Bruce D.

PY - 1979/1/1

Y1 - 1979/1/1

N2 - An assay for epoxide hydrase activity is described based on the difference in solubility between the epoxide substrate and its corresponding diol. The assay conditions for insect juvenile hormone I and an epoxide-containing juvenoid have been elucidated. In each case, the epoxide partitions into an isooctane phase while the diol partitions largely into an aqueous methanol phase. The assay is faster than chromatographic assays while showing similar or superior precision.

AB - An assay for epoxide hydrase activity is described based on the difference in solubility between the epoxide substrate and its corresponding diol. The assay conditions for insect juvenile hormone I and an epoxide-containing juvenoid have been elucidated. In each case, the epoxide partitions into an isooctane phase while the diol partitions largely into an aqueous methanol phase. The assay is faster than chromatographic assays while showing similar or superior precision.

UR - http://www.scopus.com/inward/record.url?scp=0018347287&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018347287&partnerID=8YFLogxK

U2 - 10.1016/0003-2697(79)90619-5

DO - 10.1016/0003-2697(79)90619-5

M3 - Article

C2 - 426275

AN - SCOPUS:0018347287

VL - 92

SP - 16

EP - 21

JO - Analytical Biochemistry

JF - Analytical Biochemistry

SN - 0003-2697

IS - 1

ER -