A partition assay for epoxide hydrases acting on insect juvenile hormone and an epoxide-containing juvenoid

Susanne M. Mumby; Bruce D. Hammock

doi:10.1016/0003-2697(79)90619-5

A partition assay for epoxide hydrases acting on insect juvenile hormone and an epoxide-containing juvenoid

Susanne M. Mumby, Bruce D. Hammock

Entomology

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43 Scopus citations

Abstract

An assay for epoxide hydrase activity is described based on the difference in solubility between the epoxide substrate and its corresponding diol. The assay conditions for insect juvenile hormone I and an epoxide-containing juvenoid have been elucidated. In each case, the epoxide partitions into an isooctane phase while the diol partitions largely into an aqueous methanol phase. The assay is faster than chromatographic assays while showing similar or superior precision.

Original language	English (US)
Pages (from-to)	16-21
Number of pages	6
Journal	Analytical Biochemistry
Volume	92
Issue number	1
DOIs	https://doi.org/10.1016/0003-2697(79)90619-5
State	Published - Jan 1 1979

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ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology

Cite this

Mumby, S. M., & Hammock, B. D. (1979). A partition assay for epoxide hydrases acting on insect juvenile hormone and an epoxide-containing juvenoid. Analytical Biochemistry, 92(1), 16-21. https://doi.org/10.1016/0003-2697(79)90619-5

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Access to Document

10.1016/0003-2697(79)90619-5