A one-bead one-peptide combinatorial library method for B-cell epitope mapping

Kit Lam, Douglas Lake, Sydney E. Salmon, John Smith, Man Ling Chen, Shelly Wade, Farid Abdul-Latif, Richard J. Knapp, Zuzana Leblova, Ronald D. Ferguson, Viktor Krchnak, Nikolai F. Sepetov, Michal Lebl

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

The one-bead one-peptide combinatorial library method represents a powerful approach to the discovery of binding peptides for various macromolecular targets. It involves the synthesis of millions of peptides on beads such that each bead displays only one peptide entity. The peptide-beads that interact with a specific macromolecular target are then isolated for structure determination. We have applied this method to discovering peptide ligands for several murine monoclonal antibodies: (i) anti-β-endorphin (continuous epitope), (ii) anti-vmos peptide, (iii) antihuman insulin (discontinuous epitope), and (iv) surface immunoglobulins (μκ) of two murine B-cell lymphoma cell lines (antigen unknown).

Original languageEnglish (US)
Pages (from-to)482-493
Number of pages12
JournalMethods: A Companion to Methods in Enzymology
Volume9
Issue number3
DOIs
StatePublished - Jun 1996
Externally publishedYes

Fingerprint

B-Lymphocyte Epitopes
Epitope Mapping
Peptide Library
Peptides
Epitopes
Endorphins
Cells
B-Cell Antigen Receptors
B-Cell Lymphoma
Monoclonal Antibodies
Insulin
Ligands
Antigens
Cell Line

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

A one-bead one-peptide combinatorial library method for B-cell epitope mapping. / Lam, Kit; Lake, Douglas; Salmon, Sydney E.; Smith, John; Chen, Man Ling; Wade, Shelly; Abdul-Latif, Farid; Knapp, Richard J.; Leblova, Zuzana; Ferguson, Ronald D.; Krchnak, Viktor; Sepetov, Nikolai F.; Lebl, Michal.

In: Methods: A Companion to Methods in Enzymology, Vol. 9, No. 3, 06.1996, p. 482-493.

Research output: Contribution to journalArticle

Lam, K, Lake, D, Salmon, SE, Smith, J, Chen, ML, Wade, S, Abdul-Latif, F, Knapp, RJ, Leblova, Z, Ferguson, RD, Krchnak, V, Sepetov, NF & Lebl, M 1996, 'A one-bead one-peptide combinatorial library method for B-cell epitope mapping', Methods: A Companion to Methods in Enzymology, vol. 9, no. 3, pp. 482-493. https://doi.org/10.1006/meth.1996.0056
Lam, Kit ; Lake, Douglas ; Salmon, Sydney E. ; Smith, John ; Chen, Man Ling ; Wade, Shelly ; Abdul-Latif, Farid ; Knapp, Richard J. ; Leblova, Zuzana ; Ferguson, Ronald D. ; Krchnak, Viktor ; Sepetov, Nikolai F. ; Lebl, Michal. / A one-bead one-peptide combinatorial library method for B-cell epitope mapping. In: Methods: A Companion to Methods in Enzymology. 1996 ; Vol. 9, No. 3. pp. 482-493.
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