A novel three-dimensional model to quantify metastatic melanoma invasion

Cyrus M. Ghajar, Vinod Suresh, Shelly R. Peyton, Christopher B. Raub, Frank L. Meyskens, Steven George, Andrew J. Putnam

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Although attempts to develop any viable chemotherapeutic approaches to combat metastatic cancers have largely failed, potential genetic targets to halt metastatic progression continue to be identified. As drugs are developed to address these targets, there is a need for high-throughput systems that accurately reproduce in vivo microenvironments to gauge their efficacy. Accordingly, we have developed a three-dimensional in vitro culture system representative of the environment present upon secondary metastasis to quantitatively measure tumor cell invasion in this setting three-dimensionally. Culturing melanomas of different metastatic capacities within the system showed that each cell type invades the matrix in a manner commensurate to its known metastatic potential in vivo. Moreover, the developed quantitative schemes were put to use to characterize the effect of microenvironmental influences (i.e., matrix components, interstitial cell presence) on planar and vertical melanoma invasion. We propose this novel, quantitative system as a useful tool to assess the effects of pharmacologic and/or microenvironmental influences on tumor cell invasion at a metastatic site.

Original languageEnglish (US)
Pages (from-to)552-561
Number of pages10
JournalMolecular Cancer Therapeutics
Volume6
Issue number2
DOIs
StatePublished - Feb 1 2007

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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