A novel targeting signal for proximal clustering of the Kv2.1 K+ channel in hippocampal neurons

Seung T. Lim, Dana E. Antonucci, Robert H. Scannevin, James Trimmer

Research output: Contribution to journalArticle

180 Scopus citations

Abstract

The discrete localization of ion channels is a critical determinant of neuronal excitability. We show here that the dendritic K+ channels Kv2.1 and Kv2.2 were differentially targeted in cultured hippocampal neurons. Kv2.1 was found in high-density clusters on the soma and proximal dendrites, while Kv2.2 was uniformly distributed throughout the soma and dendrites. Chimeras revealed a proximal restriction and clustering domain on the cytoplasmic tail of Kv2.1. Truncations and internal deletions revealed a 26-amino acid targeting signal within which four residues were critical for localization. This signal is not related to other known sequences for neuronal and epithelial membrane protein targeting and represents a novel cytoplasmic signal responsible for proximal restriction and clustering.

Original languageEnglish (US)
Pages (from-to)385-397
Number of pages13
JournalNeuron
Volume25
Issue number2
StatePublished - 2000
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)

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    Lim, S. T., Antonucci, D. E., Scannevin, R. H., & Trimmer, J. (2000). A novel targeting signal for proximal clustering of the Kv2.1 K+ channel in hippocampal neurons. Neuron, 25(2), 385-397.