A novel targeting signal for proximal clustering of the Kv2.1 K+ channel in hippocampal neurons

Seung T. Lim, Dana E. Antonucci, Robert H. Scannevin, James Trimmer

Research output: Contribution to journalArticle

173 Citations (Scopus)

Abstract

The discrete localization of ion channels is a critical determinant of neuronal excitability. We show here that the dendritic K+ channels Kv2.1 and Kv2.2 were differentially targeted in cultured hippocampal neurons. Kv2.1 was found in high-density clusters on the soma and proximal dendrites, while Kv2.2 was uniformly distributed throughout the soma and dendrites. Chimeras revealed a proximal restriction and clustering domain on the cytoplasmic tail of Kv2.1. Truncations and internal deletions revealed a 26-amino acid targeting signal within which four residues were critical for localization. This signal is not related to other known sequences for neuronal and epithelial membrane protein targeting and represents a novel cytoplasmic signal responsible for proximal restriction and clustering.

Original languageEnglish (US)
Pages (from-to)385-397
Number of pages13
JournalNeuron
Volume25
Issue number2
StatePublished - 2000
Externally publishedYes

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Carisoprodol
Dendrites
Cluster Analysis
Neurons
Protein Transport
Ion Channels
Membrane Proteins
Amino Acids

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Lim, S. T., Antonucci, D. E., Scannevin, R. H., & Trimmer, J. (2000). A novel targeting signal for proximal clustering of the Kv2.1 K+ channel in hippocampal neurons. Neuron, 25(2), 385-397.

A novel targeting signal for proximal clustering of the Kv2.1 K+ channel in hippocampal neurons. / Lim, Seung T.; Antonucci, Dana E.; Scannevin, Robert H.; Trimmer, James.

In: Neuron, Vol. 25, No. 2, 2000, p. 385-397.

Research output: Contribution to journalArticle

Lim, ST, Antonucci, DE, Scannevin, RH & Trimmer, J 2000, 'A novel targeting signal for proximal clustering of the Kv2.1 K+ channel in hippocampal neurons', Neuron, vol. 25, no. 2, pp. 385-397.
Lim ST, Antonucci DE, Scannevin RH, Trimmer J. A novel targeting signal for proximal clustering of the Kv2.1 K+ channel in hippocampal neurons. Neuron. 2000;25(2):385-397.
Lim, Seung T. ; Antonucci, Dana E. ; Scannevin, Robert H. ; Trimmer, James. / A novel targeting signal for proximal clustering of the Kv2.1 K+ channel in hippocampal neurons. In: Neuron. 2000 ; Vol. 25, No. 2. pp. 385-397.
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