Abstract
The discrete localization of ion channels is a critical determinant of neuronal excitability. We show here that the dendritic K+ channels Kv2.1 and Kv2.2 were differentially targeted in cultured hippocampal neurons. Kv2.1 was found in high-density clusters on the soma and proximal dendrites, while Kv2.2 was uniformly distributed throughout the soma and dendrites. Chimeras revealed a proximal restriction and clustering domain on the cytoplasmic tail of Kv2.1. Truncations and internal deletions revealed a 26-amino acid targeting signal within which four residues were critical for localization. This signal is not related to other known sequences for neuronal and epithelial membrane protein targeting and represents a novel cytoplasmic signal responsible for proximal restriction and clustering.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 385-397 |
| Number of pages | 13 |
| Journal | Neuron |
| Volume | 25 |
| Issue number | 2 |
| State | Published - 2000 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Neuroscience(all)
Cite this
A novel targeting signal for proximal clustering of the Kv2.1 K+ channel in hippocampal neurons. / Lim, Seung T.; Antonucci, Dana E.; Scannevin, Robert H.; Trimmer, James.
In: Neuron, Vol. 25, No. 2, 2000, p. 385-397.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A novel targeting signal for proximal clustering of the Kv2.1 K+ channel in hippocampal neurons
AU - Lim, Seung T.
AU - Antonucci, Dana E.
AU - Scannevin, Robert H.
AU - Trimmer, James
PY - 2000
Y1 - 2000
N2 - The discrete localization of ion channels is a critical determinant of neuronal excitability. We show here that the dendritic K+ channels Kv2.1 and Kv2.2 were differentially targeted in cultured hippocampal neurons. Kv2.1 was found in high-density clusters on the soma and proximal dendrites, while Kv2.2 was uniformly distributed throughout the soma and dendrites. Chimeras revealed a proximal restriction and clustering domain on the cytoplasmic tail of Kv2.1. Truncations and internal deletions revealed a 26-amino acid targeting signal within which four residues were critical for localization. This signal is not related to other known sequences for neuronal and epithelial membrane protein targeting and represents a novel cytoplasmic signal responsible for proximal restriction and clustering.
AB - The discrete localization of ion channels is a critical determinant of neuronal excitability. We show here that the dendritic K+ channels Kv2.1 and Kv2.2 were differentially targeted in cultured hippocampal neurons. Kv2.1 was found in high-density clusters on the soma and proximal dendrites, while Kv2.2 was uniformly distributed throughout the soma and dendrites. Chimeras revealed a proximal restriction and clustering domain on the cytoplasmic tail of Kv2.1. Truncations and internal deletions revealed a 26-amino acid targeting signal within which four residues were critical for localization. This signal is not related to other known sequences for neuronal and epithelial membrane protein targeting and represents a novel cytoplasmic signal responsible for proximal restriction and clustering.
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M3 - Article
C2 - 10719893
AN - SCOPUS:0033757858
VL - 25
SP - 385
EP - 397
JO - Neuron
JF - Neuron
SN - 0896-6273
IS - 2
ER -