A novel specific small molecule peptide for small cell lung cancer

Lin Lang Guo, Ying Quo, Derick H Lau, Sha Xiao, Yin Chao Xu, Hong Shen

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Screen small molecule peptide specific binding to small cell lung cancer cell (DMS53) was screened by using the "one-bead one-peptide" combinatorial technology. Thirty two positive beads binding to DMS53 were totally obtained after primary screening. Consensus peptide sequences of cXNGRXXc and cNGRXXXc were identified by amino acid sequencing in ten beads. Three representative peptides were re-synthesized on beads. Secondary screening showed that cell adhesion percentage of cFNGRQQc to DMSS was higher than the other two peptides. cFNGRQQc was further studied for cell specificity, alanine scanning and site-directed deletion. The results showed that cFNGRQQc is specific for promoting cell adhesion to DMS53 but not to other human cell lines. Both motif of -NGR- and the length of six peptide of cFNGRQQc structure are important for DMS53 attachment, In an antibody or peptide blocking assay, cell adhesion of DMS53 to peptide bead was not inhibited by antibodies or peptides including anti-integrin, E-cadherin, NCAM and ICAM. The binding site on DMS53 surface for cFNGRQQc peptide need to be proven in the future.

Original languageEnglish (US)
Pages (from-to)562-566
Number of pages5
JournalProgress in Biochemistry and Biophysics
Volume33
Issue number6
StatePublished - Jun 2006

Keywords

  • Combinatorial chemistry
  • Small cell lung cancer
  • Small molecule peptide

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

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  • Cite this

    Guo, L. L., Quo, Y., Lau, D. H., Xiao, S., Xu, Y. C., & Shen, H. (2006). A novel specific small molecule peptide for small cell lung cancer. Progress in Biochemistry and Biophysics, 33(6), 562-566.