Abstract
To screen small molecule peptide specific binding to non-small cell lung cancer cell (A549) using the "one-bead one-peptide" combinatorial library. Twenty-nine positive beads binding to A549 cell were totally obtained after primary screening. Consensus peptide sequence of -NGXG- was identified by amino acid sequencing in ten beads. Peptide cNGQGEQc was re-synthesized on beads and further studied for its cell specificity, alanine scanning and site-directed deletion. The results showed that cNGQGEQc is specific for cell attachment to non-small cell lung cancer cells including A549, Calu-I and H178, but not to other tumor cell lines. Both motif of -NGXG- and the length of six peptides are very important for A549 adhesion. Peptide cNGQGEQc labeled with FITC can specifically bind to A549 cell. In a blocking assay with anti-integrin antibodies(α 1-6, αv/β1-5), cell adhesion of A549 to peptide beads was obviously inhibited by integrin α3 combining with any αsubunits. Results suggested that small molecule peptide cNGQGEQc can bind specifically to non-small cell lung cancer cell A549 via integrin α3 on cell surface.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1080-1085 |
| Number of pages | 6 |
| Journal | Progress in Biochemistry and Biophysics |
| Volume | 34 |
| Issue number | 10 |
| State | Published - Oct 2007 |
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Keywords
- Combinatorial chemistry
- Non-small cell lung cancer
- Small molecule peptide
ASJC Scopus subject areas
- Biochemistry
- Biophysics
Cite this
A novel specific small molecule peptide for non-small cell lung cancer cell A549. / Guo, Lin Lang; Guo, Ying; Lau, Derick H; Xu, Yin Chao.
In: Progress in Biochemistry and Biophysics, Vol. 34, No. 10, 10.2007, p. 1080-1085.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A novel specific small molecule peptide for non-small cell lung cancer cell A549
AU - Guo, Lin Lang
AU - Guo, Ying
AU - Lau, Derick H
AU - Xu, Yin Chao
PY - 2007/10
Y1 - 2007/10
N2 - To screen small molecule peptide specific binding to non-small cell lung cancer cell (A549) using the "one-bead one-peptide" combinatorial library. Twenty-nine positive beads binding to A549 cell were totally obtained after primary screening. Consensus peptide sequence of -NGXG- was identified by amino acid sequencing in ten beads. Peptide cNGQGEQc was re-synthesized on beads and further studied for its cell specificity, alanine scanning and site-directed deletion. The results showed that cNGQGEQc is specific for cell attachment to non-small cell lung cancer cells including A549, Calu-I and H178, but not to other tumor cell lines. Both motif of -NGXG- and the length of six peptides are very important for A549 adhesion. Peptide cNGQGEQc labeled with FITC can specifically bind to A549 cell. In a blocking assay with anti-integrin antibodies(α 1-6, αv/β1-5), cell adhesion of A549 to peptide beads was obviously inhibited by integrin α3 combining with any αsubunits. Results suggested that small molecule peptide cNGQGEQc can bind specifically to non-small cell lung cancer cell A549 via integrin α3 on cell surface.
AB - To screen small molecule peptide specific binding to non-small cell lung cancer cell (A549) using the "one-bead one-peptide" combinatorial library. Twenty-nine positive beads binding to A549 cell were totally obtained after primary screening. Consensus peptide sequence of -NGXG- was identified by amino acid sequencing in ten beads. Peptide cNGQGEQc was re-synthesized on beads and further studied for its cell specificity, alanine scanning and site-directed deletion. The results showed that cNGQGEQc is specific for cell attachment to non-small cell lung cancer cells including A549, Calu-I and H178, but not to other tumor cell lines. Both motif of -NGXG- and the length of six peptides are very important for A549 adhesion. Peptide cNGQGEQc labeled with FITC can specifically bind to A549 cell. In a blocking assay with anti-integrin antibodies(α 1-6, αv/β1-5), cell adhesion of A549 to peptide beads was obviously inhibited by integrin α3 combining with any αsubunits. Results suggested that small molecule peptide cNGQGEQc can bind specifically to non-small cell lung cancer cell A549 via integrin α3 on cell surface.
KW - Combinatorial chemistry
KW - Non-small cell lung cancer
KW - Small molecule peptide
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UR - http://www.scopus.com/inward/citedby.url?scp=39149125110&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:39149125110
VL - 34
SP - 1080
EP - 1085
JO - Progress in Biochemistry and Biophysics
JF - Progress in Biochemistry and Biophysics
SN - 1000-3282
IS - 10
ER -