A novel pulmonary polyomavirus in alpacas (Vicugna pacos)

Florante N. Dela Cruz, Linlin Li, Eric Delwart, Patricia Pesavento

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Viral metagenomic analysis detected a novel polyomavirus in a 6-month old female alpaca (Vicugna pacos) euthanized after a diagnosis of disseminated lymphosarcoma. The viral genome was fully sequenced, found to be similar to other polyomaviruses in gene architecture and provisionally named Alpaca polyomavirus or AlPyV. Viral nucleic acid was detected by PCR in venous blood, spleen, thymus, and lung. AlPyV phylogenetically clustered in the “Wuki” group of PyVs, which includes WU and KI polyomaviruses, commonly found in human respiratory samples. In an ISH analysis of 17 alpaca necropsies, 7 had detectable virus within the lung. In animals without pneumonia, probe hybridization was restricted to the nuclei of scattered individual bronchiolar epithelial cells. Three of the ISH positive alpacas had interstitial pneumonia of unknown origin, and in these animals there was viral nucleic acid detected in bronchiolar epithelium, type II pneumocytes, and alveolar macrophages. The pattern of AlPyV distribution is consistent with a persistent respiratory virus that has a possible role in respiratory disease.

Original languageEnglish (US)
Pages (from-to)49-55
Number of pages7
JournalVeterinary Microbiology
Volume201
DOIs
StatePublished - Mar 1 2017

Fingerprint

Polyomavirus
New World Camelids
alpacas
Polyomaviridae
lungs
Lung
nucleic acids
pneumonia
Nucleic Acids
pneumocytes
viruses
lymphosarcoma
Viruses
Alveolar Epithelial Cells
Metagenomics
respiratory tract diseases
Viral Genome
Interstitial Lung Diseases
Alveolar Macrophages
necropsy

Keywords

  • Alpaca
  • Metagenomics
  • Polyomavirus
  • Respiratory

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

A novel pulmonary polyomavirus in alpacas (Vicugna pacos). / Dela Cruz, Florante N.; Li, Linlin; Delwart, Eric; Pesavento, Patricia.

In: Veterinary Microbiology, Vol. 201, 01.03.2017, p. 49-55.

Research output: Contribution to journalArticle

Dela Cruz, Florante N. ; Li, Linlin ; Delwart, Eric ; Pesavento, Patricia. / A novel pulmonary polyomavirus in alpacas (Vicugna pacos). In: Veterinary Microbiology. 2017 ; Vol. 201. pp. 49-55.
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