Abstract
Viral metagenomic analysis detected a novel polyomavirus in a 6-month old female alpaca (Vicugna pacos) euthanized after a diagnosis of disseminated lymphosarcoma. The viral genome was fully sequenced, found to be similar to other polyomaviruses in gene architecture and provisionally named Alpaca polyomavirus or AlPyV. Viral nucleic acid was detected by PCR in venous blood, spleen, thymus, and lung. AlPyV phylogenetically clustered in the “Wuki” group of PyVs, which includes WU and KI polyomaviruses, commonly found in human respiratory samples. In an ISH analysis of 17 alpaca necropsies, 7 had detectable virus within the lung. In animals without pneumonia, probe hybridization was restricted to the nuclei of scattered individual bronchiolar epithelial cells. Three of the ISH positive alpacas had interstitial pneumonia of unknown origin, and in these animals there was viral nucleic acid detected in bronchiolar epithelium, type II pneumocytes, and alveolar macrophages. The pattern of AlPyV distribution is consistent with a persistent respiratory virus that has a possible role in respiratory disease.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 49-55 |
| Number of pages | 7 |
| Journal | Veterinary Microbiology |
| Volume | 201 |
| DOIs | |
| State | Published - Mar 1 2017 |
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Keywords
- Alpaca
- Metagenomics
- Polyomavirus
- Respiratory
ASJC Scopus subject areas
- Microbiology
- veterinary(all)
Cite this
A novel pulmonary polyomavirus in alpacas (Vicugna pacos). / Dela Cruz, Florante N.; Li, Linlin; Delwart, Eric; Pesavento, Patricia.
In: Veterinary Microbiology, Vol. 201, 01.03.2017, p. 49-55.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A novel pulmonary polyomavirus in alpacas (Vicugna pacos)
AU - Dela Cruz, Florante N.
AU - Li, Linlin
AU - Delwart, Eric
AU - Pesavento, Patricia
PY - 2017/3/1
Y1 - 2017/3/1
N2 - Viral metagenomic analysis detected a novel polyomavirus in a 6-month old female alpaca (Vicugna pacos) euthanized after a diagnosis of disseminated lymphosarcoma. The viral genome was fully sequenced, found to be similar to other polyomaviruses in gene architecture and provisionally named Alpaca polyomavirus or AlPyV. Viral nucleic acid was detected by PCR in venous blood, spleen, thymus, and lung. AlPyV phylogenetically clustered in the “Wuki” group of PyVs, which includes WU and KI polyomaviruses, commonly found in human respiratory samples. In an ISH analysis of 17 alpaca necropsies, 7 had detectable virus within the lung. In animals without pneumonia, probe hybridization was restricted to the nuclei of scattered individual bronchiolar epithelial cells. Three of the ISH positive alpacas had interstitial pneumonia of unknown origin, and in these animals there was viral nucleic acid detected in bronchiolar epithelium, type II pneumocytes, and alveolar macrophages. The pattern of AlPyV distribution is consistent with a persistent respiratory virus that has a possible role in respiratory disease.
AB - Viral metagenomic analysis detected a novel polyomavirus in a 6-month old female alpaca (Vicugna pacos) euthanized after a diagnosis of disseminated lymphosarcoma. The viral genome was fully sequenced, found to be similar to other polyomaviruses in gene architecture and provisionally named Alpaca polyomavirus or AlPyV. Viral nucleic acid was detected by PCR in venous blood, spleen, thymus, and lung. AlPyV phylogenetically clustered in the “Wuki” group of PyVs, which includes WU and KI polyomaviruses, commonly found in human respiratory samples. In an ISH analysis of 17 alpaca necropsies, 7 had detectable virus within the lung. In animals without pneumonia, probe hybridization was restricted to the nuclei of scattered individual bronchiolar epithelial cells. Three of the ISH positive alpacas had interstitial pneumonia of unknown origin, and in these animals there was viral nucleic acid detected in bronchiolar epithelium, type II pneumocytes, and alveolar macrophages. The pattern of AlPyV distribution is consistent with a persistent respiratory virus that has a possible role in respiratory disease.
KW - Alpaca
KW - Metagenomics
KW - Polyomavirus
KW - Respiratory
UR - http://www.scopus.com/inward/record.url?scp=85009863252&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85009863252&partnerID=8YFLogxK
U2 - 10.1016/j.vetmic.2017.01.005
DO - 10.1016/j.vetmic.2017.01.005
M3 - Article
C2 - 28284622
AN - SCOPUS:85009863252
VL - 201
SP - 49
EP - 55
JO - Veterinary Microbiology
JF - Veterinary Microbiology
SN - 0378-1135
ER -