A novel protein polymorphism differentiates the California serotype of infectious bronchitis from other serotypes common to California

James Case, Karen W. Sverlow, Bruce J. Reynolds

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The California (Cal) serotype of infectious bronchitis virus (IBV) was isolated from layer flocks in southern California in the early 1980s. Since then, it has spread to the broiler-producing regions of central California, where it has been implicated in respiratory disease outbreaks in vaccinated flocks. Lack of a procedure for quickly identifying IBV serotypes in commercial chicken flocks has prevented the causal association of the IBV Cal serotype with respiratory disease outbreaks. A protein polymorphism has been identified in the matrix protein of the Cal serotype; it appears to be unique among other common serotypes of infectious bronchitis virus found in California. This polymorphism can be identified on western blots using raw or concentrated infectious allantoic fluid as the source material. Identification of the Cal serotype and of serotypes in the Mass and Conn groups can be performed rapidly using field samples from suspect flocks. The identification of this polymorphism provides an alternative method for the rapid identification of the Cal serotype of IBV.

Original languageEnglish (US)
Pages (from-to)149-155
Number of pages7
JournalJournal of Veterinary Diagnostic Investigation
Volume9
Issue number2
DOIs
StatePublished - Jan 1 1997

Fingerprint

infectious bronchitis
Bronchitis
polymorphism
Infectious bronchitis virus
serotypes
flocks
Proteins
proteins
respiratory tract diseases
Disease Outbreaks
allantoic fluid
Serogroup
rapid methods
Chickens
Western blotting
broiler chickens
Western Blotting
chickens

ASJC Scopus subject areas

  • veterinary(all)

Cite this

A novel protein polymorphism differentiates the California serotype of infectious bronchitis from other serotypes common to California. / Case, James; Sverlow, Karen W.; Reynolds, Bruce J.

In: Journal of Veterinary Diagnostic Investigation, Vol. 9, No. 2, 01.01.1997, p. 149-155.

Research output: Contribution to journalArticle

@article{bae87c4c56294bacad3581dbe18bbb2a,
title = "A novel protein polymorphism differentiates the California serotype of infectious bronchitis from other serotypes common to California",
abstract = "The California (Cal) serotype of infectious bronchitis virus (IBV) was isolated from layer flocks in southern California in the early 1980s. Since then, it has spread to the broiler-producing regions of central California, where it has been implicated in respiratory disease outbreaks in vaccinated flocks. Lack of a procedure for quickly identifying IBV serotypes in commercial chicken flocks has prevented the causal association of the IBV Cal serotype with respiratory disease outbreaks. A protein polymorphism has been identified in the matrix protein of the Cal serotype; it appears to be unique among other common serotypes of infectious bronchitis virus found in California. This polymorphism can be identified on western blots using raw or concentrated infectious allantoic fluid as the source material. Identification of the Cal serotype and of serotypes in the Mass and Conn groups can be performed rapidly using field samples from suspect flocks. The identification of this polymorphism provides an alternative method for the rapid identification of the Cal serotype of IBV.",
author = "James Case and Sverlow, {Karen W.} and Reynolds, {Bruce J.}",
year = "1997",
month = "1",
day = "1",
doi = "10.1177/104063879700900207",
language = "English (US)",
volume = "9",
pages = "149--155",
journal = "Journal of Veterinary Diagnostic Investigation",
issn = "1040-6387",
publisher = "American Association of Veterinary Laboratory Diagnosticians",
number = "2",

}

TY - JOUR

T1 - A novel protein polymorphism differentiates the California serotype of infectious bronchitis from other serotypes common to California

AU - Case, James

AU - Sverlow, Karen W.

AU - Reynolds, Bruce J.

PY - 1997/1/1

Y1 - 1997/1/1

N2 - The California (Cal) serotype of infectious bronchitis virus (IBV) was isolated from layer flocks in southern California in the early 1980s. Since then, it has spread to the broiler-producing regions of central California, where it has been implicated in respiratory disease outbreaks in vaccinated flocks. Lack of a procedure for quickly identifying IBV serotypes in commercial chicken flocks has prevented the causal association of the IBV Cal serotype with respiratory disease outbreaks. A protein polymorphism has been identified in the matrix protein of the Cal serotype; it appears to be unique among other common serotypes of infectious bronchitis virus found in California. This polymorphism can be identified on western blots using raw or concentrated infectious allantoic fluid as the source material. Identification of the Cal serotype and of serotypes in the Mass and Conn groups can be performed rapidly using field samples from suspect flocks. The identification of this polymorphism provides an alternative method for the rapid identification of the Cal serotype of IBV.

AB - The California (Cal) serotype of infectious bronchitis virus (IBV) was isolated from layer flocks in southern California in the early 1980s. Since then, it has spread to the broiler-producing regions of central California, where it has been implicated in respiratory disease outbreaks in vaccinated flocks. Lack of a procedure for quickly identifying IBV serotypes in commercial chicken flocks has prevented the causal association of the IBV Cal serotype with respiratory disease outbreaks. A protein polymorphism has been identified in the matrix protein of the Cal serotype; it appears to be unique among other common serotypes of infectious bronchitis virus found in California. This polymorphism can be identified on western blots using raw or concentrated infectious allantoic fluid as the source material. Identification of the Cal serotype and of serotypes in the Mass and Conn groups can be performed rapidly using field samples from suspect flocks. The identification of this polymorphism provides an alternative method for the rapid identification of the Cal serotype of IBV.

UR - http://www.scopus.com/inward/record.url?scp=0031111049&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031111049&partnerID=8YFLogxK

U2 - 10.1177/104063879700900207

DO - 10.1177/104063879700900207

M3 - Article

C2 - 9211233

AN - SCOPUS:0031111049

VL - 9

SP - 149

EP - 155

JO - Journal of Veterinary Diagnostic Investigation

JF - Journal of Veterinary Diagnostic Investigation

SN - 1040-6387

IS - 2

ER -