A novel oxysterol promotes bone regeneration in rabbit cranial bone defects

Akishige Hokugo, Sarah Sorice, Farhad Parhami, Anisa Yalom, Andrew Li, Patricia Zuk, Reza Jarrahy

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Bone morphogenetic proteins (BMPs) have played a central role in the development of regenerative therapies for bone reconstruction. However, the high cost and side-effect profile of BMPs limits their broad application. Oxysterols, naturally occurring products of cholesterol oxidation, are promising osteogenic agents alternative to BMPs. The osteogenic capacity of these non-toxic and relatively inexpensive molecules has been documented in rodent models. We studied the impact of Oxy49, a novel oxysterol analogue, on the osteogenic differentiation of rabbit bone marrow stromal cells (BMSCs). Moreover, we evaluated the capacity for in vivo bone regeneration with Oxy49 in rabbit cranial bone defects. We found that rabbit BMSCs treated with Oxy49 demonstrated differentiation along osteogenic pathways, and that complete bone regeneration occurred when cranial defects were treated with Oxy49. Collectively, these results demonstrate that Oxy49 has the ability to induce osteogenic differentiation in rabbit BMSCs with an efficacy comparable to that of BMP-2 and to promote significant bone regeneration in cranial defects. Oxysterols may be a viable novel agent in bone tissue engineering.

Original languageEnglish (US)
Pages (from-to)591-599
Number of pages9
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume10
Issue number7
DOIs
StatePublished - Jul 1 2016
Externally publishedYes

Fingerprint

Bone Regeneration
Bone Morphogenetic Proteins
Bone
Mesenchymal Stromal Cells
Rabbits
Bone and Bones
Defects
Bone Morphogenetic Protein 2
Tissue Engineering
Rodentia
Proteins
Cholesterol
Costs and Cost Analysis
Oxysterols
Tissue engineering
Oxidation
Molecules
Therapeutics

Keywords

  • bone regeneration
  • cranial bone defect
  • Hedgehog signalling
  • osteogenesis
  • oxysterol
  • rabbit

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

A novel oxysterol promotes bone regeneration in rabbit cranial bone defects. / Hokugo, Akishige; Sorice, Sarah; Parhami, Farhad; Yalom, Anisa; Li, Andrew; Zuk, Patricia; Jarrahy, Reza.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 10, No. 7, 01.07.2016, p. 591-599.

Research output: Contribution to journalArticle

Hokugo, Akishige ; Sorice, Sarah ; Parhami, Farhad ; Yalom, Anisa ; Li, Andrew ; Zuk, Patricia ; Jarrahy, Reza. / A novel oxysterol promotes bone regeneration in rabbit cranial bone defects. In: Journal of Tissue Engineering and Regenerative Medicine. 2016 ; Vol. 10, No. 7. pp. 591-599.
@article{f794a9d43d2c49d890df1fa0dad06b98,
title = "A novel oxysterol promotes bone regeneration in rabbit cranial bone defects",
abstract = "Bone morphogenetic proteins (BMPs) have played a central role in the development of regenerative therapies for bone reconstruction. However, the high cost and side-effect profile of BMPs limits their broad application. Oxysterols, naturally occurring products of cholesterol oxidation, are promising osteogenic agents alternative to BMPs. The osteogenic capacity of these non-toxic and relatively inexpensive molecules has been documented in rodent models. We studied the impact of Oxy49, a novel oxysterol analogue, on the osteogenic differentiation of rabbit bone marrow stromal cells (BMSCs). Moreover, we evaluated the capacity for in vivo bone regeneration with Oxy49 in rabbit cranial bone defects. We found that rabbit BMSCs treated with Oxy49 demonstrated differentiation along osteogenic pathways, and that complete bone regeneration occurred when cranial defects were treated with Oxy49. Collectively, these results demonstrate that Oxy49 has the ability to induce osteogenic differentiation in rabbit BMSCs with an efficacy comparable to that of BMP-2 and to promote significant bone regeneration in cranial defects. Oxysterols may be a viable novel agent in bone tissue engineering.",
keywords = "bone regeneration, cranial bone defect, Hedgehog signalling, osteogenesis, oxysterol, rabbit",
author = "Akishige Hokugo and Sarah Sorice and Farhad Parhami and Anisa Yalom and Andrew Li and Patricia Zuk and Reza Jarrahy",
year = "2016",
month = "7",
day = "1",
doi = "10.1002/term.1799",
language = "English (US)",
volume = "10",
pages = "591--599",
journal = "Journal of Tissue Engineering and Regenerative Medicine",
issn = "1932-6254",
publisher = "John Wiley and Sons Ltd",
number = "7",

}

TY - JOUR

T1 - A novel oxysterol promotes bone regeneration in rabbit cranial bone defects

AU - Hokugo, Akishige

AU - Sorice, Sarah

AU - Parhami, Farhad

AU - Yalom, Anisa

AU - Li, Andrew

AU - Zuk, Patricia

AU - Jarrahy, Reza

PY - 2016/7/1

Y1 - 2016/7/1

N2 - Bone morphogenetic proteins (BMPs) have played a central role in the development of regenerative therapies for bone reconstruction. However, the high cost and side-effect profile of BMPs limits their broad application. Oxysterols, naturally occurring products of cholesterol oxidation, are promising osteogenic agents alternative to BMPs. The osteogenic capacity of these non-toxic and relatively inexpensive molecules has been documented in rodent models. We studied the impact of Oxy49, a novel oxysterol analogue, on the osteogenic differentiation of rabbit bone marrow stromal cells (BMSCs). Moreover, we evaluated the capacity for in vivo bone regeneration with Oxy49 in rabbit cranial bone defects. We found that rabbit BMSCs treated with Oxy49 demonstrated differentiation along osteogenic pathways, and that complete bone regeneration occurred when cranial defects were treated with Oxy49. Collectively, these results demonstrate that Oxy49 has the ability to induce osteogenic differentiation in rabbit BMSCs with an efficacy comparable to that of BMP-2 and to promote significant bone regeneration in cranial defects. Oxysterols may be a viable novel agent in bone tissue engineering.

AB - Bone morphogenetic proteins (BMPs) have played a central role in the development of regenerative therapies for bone reconstruction. However, the high cost and side-effect profile of BMPs limits their broad application. Oxysterols, naturally occurring products of cholesterol oxidation, are promising osteogenic agents alternative to BMPs. The osteogenic capacity of these non-toxic and relatively inexpensive molecules has been documented in rodent models. We studied the impact of Oxy49, a novel oxysterol analogue, on the osteogenic differentiation of rabbit bone marrow stromal cells (BMSCs). Moreover, we evaluated the capacity for in vivo bone regeneration with Oxy49 in rabbit cranial bone defects. We found that rabbit BMSCs treated with Oxy49 demonstrated differentiation along osteogenic pathways, and that complete bone regeneration occurred when cranial defects were treated with Oxy49. Collectively, these results demonstrate that Oxy49 has the ability to induce osteogenic differentiation in rabbit BMSCs with an efficacy comparable to that of BMP-2 and to promote significant bone regeneration in cranial defects. Oxysterols may be a viable novel agent in bone tissue engineering.

KW - bone regeneration

KW - cranial bone defect

KW - Hedgehog signalling

KW - osteogenesis

KW - oxysterol

KW - rabbit

UR - http://www.scopus.com/inward/record.url?scp=84883181211&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84883181211&partnerID=8YFLogxK

U2 - 10.1002/term.1799

DO - 10.1002/term.1799

M3 - Article

C2 - 23997014

AN - SCOPUS:84883181211

VL - 10

SP - 591

EP - 599

JO - Journal of Tissue Engineering and Regenerative Medicine

JF - Journal of Tissue Engineering and Regenerative Medicine

SN - 1932-6254

IS - 7

ER -