A novel nonopioid action of enkephalins: Competitive inhibition of the mammalian brain high affinity L-proline transporter

Robert T. Fremeau, Maria Velaz-Faircloth, Joshua W. Miller, Victor A. Henzi, Susan M. Cohen, J. Victor Nadler, Saad Shafqat, Randy D. Blakely, Barbara Domin

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Abstract

The high affinity L-proline transporter (PROT) is a member of the family of Na+ (and Cl-)-dependent plasma membrane transport proteins that comprises transporters for several neurotransmitters, osmolytes, and metabolites. The brain-specific expression of PROT in a subset of putative glutamatergic pathways implies a specialized function for this novel transporter and its presumed natural substrate L-proline in excitatory synaptic transmission. However, definitive studies of the physiological role(s) of high affinity L-proline uptake have been precluded by the lack of specific uptake inhibitors. Here, we report that Leu- and Met-enkephalin and their des-tyrosyl derivatives potently and selectively inhibited high affinity L-proline uptake in rat hippocampal synaptosomes and in PROT- transfected HeLa cells. High concentrations of the opiate receptor antagonist naltrexone did not block the inhibitory actions of these peptides, arguing against an involvement of opioid receptors. Des-tyrosyl-Leu-enkephalin elevated the apparent K(m) of L-proline transport in transfected HeLa cells without altering the V(max). PROT-transfected HeLa cells did not accumulate [3H]Leu-enkephalin above background levels, demonstrating that enkephalins are not substrates for PROT. These findings indicate that enkephalins competitively inhibit mammalian brain PROT through a direct interaction with the transporter protein at or near the L-proline binding site. The high potency and specificity of des-tyrosyl-Leu-enkephalin make this compound a useful tool for elucidating the structure-function properties and physiological role(s) of PROT.

Original languageEnglish (US)
Pages (from-to)1033-1041
Number of pages9
JournalMolecular Pharmacology
Volume49
Issue number6
StatePublished - Jun 1996
Externally publishedYes

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ASJC Scopus subject areas

  • Pharmacology

Cite this

Fremeau, R. T., Velaz-Faircloth, M., Miller, J. W., Henzi, V. A., Cohen, S. M., Nadler, J. V., Shafqat, S., Blakely, R. D., & Domin, B. (1996). A novel nonopioid action of enkephalins: Competitive inhibition of the mammalian brain high affinity L-proline transporter. Molecular Pharmacology, 49(6), 1033-1041.