A novel murine β-defensin expressed in tongue, esophagus, and trachea*

Hong Peng Jia, Stephen A. Wowk, Brian C. Schutte, Sarah K. Lee, Andrea Vivado, Brian F. Tack, Charles L Bevins, Paul B. McCray

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Abstract

β-Defensins are broad spectrum antimicrobial peptides expressed at epithelial surfaces. Two human β-defensins, HBD-1 and HBD-2, have been identified. In the lung, HBD-2 is an inducible product of airway epithelia and may play a role in innate mucosal defenses. We recently characterized rat homologs (RBD-1, RBD-2) of the human genes and used these sequences to identify novel mouse genes. Mouse β-defensin-4 (MBD-4) was amplified from lung cDNA using polymerase chain reaction primers designed from conserved sequences of RBD-2 and HBD-2. A full-length cDNA was cloned which encodes a putative peptide with the sequence MRIHYLLFTFLLVLLSPLAAFTQIINNPITCMTNGAICWGPCPTAFRQIGNCGHFKVRCCKIR. The peptide shares ~40% identity with HBD-2. MBD-4 mRNA was expressed in the esophagus, tongue, and trachea but not in any of 20 other tissues surveyed. Cloning of the genomic sequence of MBD-4 revealed two nearly (>99%) identical sequences encoding MBD-4 and the presence of numerous additional highly similar genomic sequences. Radiation hybrid mapping localized this gene to a region of chromosome 8 near several other defensins, MBD-2, MBD-3, and α-defensins (cryptdins) -3 and -17, consistent with a gene cluster. Our genomic cloning and mapping data suggest that there is a large β-defensin gene family in mice. Identification of murine β-defensins provides an opportunity to understand further the role of these peptides in host defense through animal model studies and the generation of β-defensin-deficient animals by gene targeting.

Original languageEnglish (US)
Pages (from-to)33314-33320
Number of pages7
JournalJournal of Biological Chemistry
Volume275
Issue number43
DOIs
StatePublished - Oct 27 2000
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry

Cite this

Jia, H. P., Wowk, S. A., Schutte, B. C., Lee, S. K., Vivado, A., Tack, B. F., Bevins, C. L., & McCray, P. B. (2000). A novel murine β-defensin expressed in tongue, esophagus, and trachea*. Journal of Biological Chemistry, 275(43), 33314-33320. https://doi.org/10.1074/jbc.M006603200