A novel human homeobox gene distantly related to proboscipedia is expressed in lymphoid and pancreatic tissues

Kathleen A. Harrison, Kirk M. Druey, Yasuhiro Deguchi, Joseph Tuscano, John H. Kehrl

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

A novel human homeobox gene, HB9, was isolated from a cDNA library prepared from in vitro stimulated human tonsil B lymphocytes and from a human genomic library. The HB9 gene is composed of 3 exons spread over 6 kilobases of DNA. An open reading frame of 1206 nucleotides is in frame with a diverged homeodomain. The predicted HB9 protein has a molecular mass of 41 kilodaltons and is enriched for alanine, glycine, and leucine. The HB9 homeodomain is most similar to that of the Drosophila melanogaster homeobox gene proboscipedia. Northern blot analysis of poly(A) RNA purified from the human B cell line RPMI 8226 and from activated T cells revealed a major mRNA transcript of 2.2 kilobases. Similar analysis of poly(A) RNA from a variety of adult tissues demonstrated HB9 transcripts in pancreas, small intestine, and colon. Reverse transcriptase-polymerase chain reaction was used to examine HB9 RNA transcripts in hematopoietic cell lines. HB9 RNA transcripts were most prevalent in several human B cell lines and K562 cells. In addition, transcripts were detected in RNA prepared from tonsil B cells and in situ hybridization studies localized them in the germinal center region of adult tonsil. These findings suggest the involvement of HB9 in regulating gene transcription in lymphoid and pancreatic tissues.

Original languageEnglish (US)
Pages (from-to)19968-19975
Number of pages8
JournalJournal of Biological Chemistry
Volume269
Issue number31
StatePublished - Aug 5 1994
Externally publishedYes

Fingerprint

Homeobox Genes
Lymphoid Tissue
Genes
Cells
Tissue
Palatine Tonsil
B-Lymphocytes
RNA
Messenger RNA
Cell Line
T-cells
Lymphocytes
Genomic Library
Polymerase chain reaction
RNA-Directed DNA Polymerase
Molecular mass
Transcription
Gene Library
Leucine
Germinal Center

ASJC Scopus subject areas

  • Biochemistry

Cite this

A novel human homeobox gene distantly related to proboscipedia is expressed in lymphoid and pancreatic tissues. / Harrison, Kathleen A.; Druey, Kirk M.; Deguchi, Yasuhiro; Tuscano, Joseph; Kehrl, John H.

In: Journal of Biological Chemistry, Vol. 269, No. 31, 05.08.1994, p. 19968-19975.

Research output: Contribution to journalArticle

Harrison, Kathleen A. ; Druey, Kirk M. ; Deguchi, Yasuhiro ; Tuscano, Joseph ; Kehrl, John H. / A novel human homeobox gene distantly related to proboscipedia is expressed in lymphoid and pancreatic tissues. In: Journal of Biological Chemistry. 1994 ; Vol. 269, No. 31. pp. 19968-19975.
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