A novel high-throughput scanning microscope for label-free detection of protein and small-molecule chemical microarrays

Y. Y. Fei; J. P. Landry; Y. S. Sun; X. D. Zhu; J. T. Luo; X. B. Wang; Kit Lam

doi:10.1063/1.2830286

A novel high-throughput scanning microscope for label-free detection of protein and small-molecule chemical microarrays

Y. Y. Fei, J. P. Landry, Y. S. Sun, X. D. Zhu, J. T. Luo, X. B. Wang, Kit Lam

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

We describe a novel scanning optical microscope based on a polarization-modulated nulling ellipsometry. The new microscope employs a combination of scanning mirror and sample translation and thus enables high-throughput label-free detection of biomolecular microarrays with more than 10 000 protein or small-molecule targets. For illustration, we show the image of a 2760-spot protein microarray on a functionalized glass slide obtained with such a microscope. The new scanning microscope is also capable of determining, in parallel, the real-time binding kinetics of multiple molecular species under aqueous conditions.

Original language	English (US)
Article number	013708
Journal	Review of Scientific Instruments
Volume	79
Issue number	1
DOIs	https://doi.org/10.1063/1.2830286
State	Published - 2008

ASJC Scopus subject areas

Instrumentation
Physics and Astronomy (miscellaneous)

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10.1063/1.2830286

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AU - Luo, J. T.

AU - Wang, X. B.

AU - Lam, Kit

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