Abstract
We describe a novel scanning optical microscope based on a polarization-modulated nulling ellipsometry. The new microscope employs a combination of scanning mirror and sample translation and thus enables high-throughput label-free detection of biomolecular microarrays with more than 10 000 protein or small-molecule targets. For illustration, we show the image of a 2760-spot protein microarray on a functionalized glass slide obtained with such a microscope. The new scanning microscope is also capable of determining, in parallel, the real-time binding kinetics of multiple molecular species under aqueous conditions.
| Original language | English (US) |
|---|---|
| Article number | 013708 |
| Journal | Review of Scientific Instruments |
| Volume | 79 |
| Issue number | 1 |
| DOIs | |
| State | Published - 2008 |
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ASJC Scopus subject areas
- Instrumentation
- Physics and Astronomy (miscellaneous)
Cite this
A novel high-throughput scanning microscope for label-free detection of protein and small-molecule chemical microarrays. / Fei, Y. Y.; Landry, J. P.; Sun, Y. S.; Zhu, X. D.; Luo, J. T.; Wang, X. B.; Lam, Kit.
In: Review of Scientific Instruments, Vol. 79, No. 1, 013708, 2008.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A novel high-throughput scanning microscope for label-free detection of protein and small-molecule chemical microarrays
AU - Fei, Y. Y.
AU - Landry, J. P.
AU - Sun, Y. S.
AU - Zhu, X. D.
AU - Luo, J. T.
AU - Wang, X. B.
AU - Lam, Kit
PY - 2008
Y1 - 2008
N2 - We describe a novel scanning optical microscope based on a polarization-modulated nulling ellipsometry. The new microscope employs a combination of scanning mirror and sample translation and thus enables high-throughput label-free detection of biomolecular microarrays with more than 10 000 protein or small-molecule targets. For illustration, we show the image of a 2760-spot protein microarray on a functionalized glass slide obtained with such a microscope. The new scanning microscope is also capable of determining, in parallel, the real-time binding kinetics of multiple molecular species under aqueous conditions.
AB - We describe a novel scanning optical microscope based on a polarization-modulated nulling ellipsometry. The new microscope employs a combination of scanning mirror and sample translation and thus enables high-throughput label-free detection of biomolecular microarrays with more than 10 000 protein or small-molecule targets. For illustration, we show the image of a 2760-spot protein microarray on a functionalized glass slide obtained with such a microscope. The new scanning microscope is also capable of determining, in parallel, the real-time binding kinetics of multiple molecular species under aqueous conditions.
UR - http://www.scopus.com/inward/record.url?scp=38849099624&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=38849099624&partnerID=8YFLogxK
U2 - 10.1063/1.2830286
DO - 10.1063/1.2830286
M3 - Article
C2 - 18248040
AN - SCOPUS:38849099624
VL - 79
JO - Review of Scientific Instruments
JF - Review of Scientific Instruments
SN - 0034-6748
IS - 1
M1 - 013708
ER -