Abstract
We describe a novel scanning optical microscope based on a polarization-modulated nulling ellipsometry. The new microscope employs a combination of scanning mirror and sample translation and thus enables high-throughput label-free detection of biomolecular microarrays with more than 10 000 protein or small-molecule targets. For illustration, we show the image of a 2760-spot protein microarray on a functionalized glass slide obtained with such a microscope. The new scanning microscope is also capable of determining, in parallel, the real-time binding kinetics of multiple molecular species under aqueous conditions.
Original language | English (US) |
---|---|
Article number | 013708 |
Journal | Review of Scientific Instruments |
Volume | 79 |
Issue number | 1 |
DOIs | |
State | Published - 2008 |
ASJC Scopus subject areas
- Instrumentation
- Physics and Astronomy (miscellaneous)