Abstract
We describe a novel scanning optical microscope based on a polarization-modulated nulling ellipsometry. The new microscope employs a combination of scanning mirror and sample translation and thus enables high-throughput label-free detection of biomolecular microarrays with more than 10 000 protein or small-molecule targets. For illustration, we show the image of a 2760-spot protein microarray on a functionalized glass slide obtained with such a microscope. The new scanning microscope is also capable of determining, in parallel, the real-time binding kinetics of multiple molecular species under aqueous conditions.
| Original language | English (US) |
|---|---|
| Article number | 013708 |
| Journal | Review of Scientific Instruments |
| Volume | 79 |
| Issue number | 1 |
| DOIs | |
| State | Published - 2008 |
ASJC Scopus subject areas
- Instrumentation
- Physics and Astronomy (miscellaneous)
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Fei, Y. Y., Landry, J. P., Sun, Y. S., Zhu, X. D., Luo, J. T., Wang, X. B., & Lam, K. (2008). A novel high-throughput scanning microscope for label-free detection of protein and small-molecule chemical microarrays. Review of Scientific Instruments, 79(1), [013708]. https://doi.org/10.1063/1.2830286