A novel high-throughput scanning microscope for label-free detection of protein and small-molecule chemical microarrays

Y. Y. Fei, J. P. Landry, Y. S. Sun, X. D. Zhu, J. T. Luo, X. B. Wang, Kit Lam

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

We describe a novel scanning optical microscope based on a polarization-modulated nulling ellipsometry. The new microscope employs a combination of scanning mirror and sample translation and thus enables high-throughput label-free detection of biomolecular microarrays with more than 10 000 protein or small-molecule targets. For illustration, we show the image of a 2760-spot protein microarray on a functionalized glass slide obtained with such a microscope. The new scanning microscope is also capable of determining, in parallel, the real-time binding kinetics of multiple molecular species under aqueous conditions.

Original languageEnglish (US)
Article number013708
JournalReview of Scientific Instruments
Volume79
Issue number1
DOIs
StatePublished - 2008

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Microarrays
Labels
Microscopes
microscopes
Throughput
proteins
Proteins
Scanning
Molecules
scanning
molecules
optical microscopes
chutes
ellipsometry
Ellipsometry
mirrors
Mirrors
glass
kinetics
polarization

ASJC Scopus subject areas

  • Instrumentation
  • Physics and Astronomy (miscellaneous)

Cite this

A novel high-throughput scanning microscope for label-free detection of protein and small-molecule chemical microarrays. / Fei, Y. Y.; Landry, J. P.; Sun, Y. S.; Zhu, X. D.; Luo, J. T.; Wang, X. B.; Lam, Kit.

In: Review of Scientific Instruments, Vol. 79, No. 1, 013708, 2008.

Research output: Contribution to journalArticle

Fei, Y. Y. ; Landry, J. P. ; Sun, Y. S. ; Zhu, X. D. ; Luo, J. T. ; Wang, X. B. ; Lam, Kit. / A novel high-throughput scanning microscope for label-free detection of protein and small-molecule chemical microarrays. In: Review of Scientific Instruments. 2008 ; Vol. 79, No. 1.
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