A novel high-throughput scanning microscope for label-free detection of protein and small-molecule chemical microarrays

Y. Y. Fei, J. P. Landry, Y. S. Sun, X. D. Zhu, J. T. Luo, X. B. Wang, Kit Lam

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

We describe a novel scanning optical microscope based on a polarization-modulated nulling ellipsometry. The new microscope employs a combination of scanning mirror and sample translation and thus enables high-throughput label-free detection of biomolecular microarrays with more than 10 000 protein or small-molecule targets. For illustration, we show the image of a 2760-spot protein microarray on a functionalized glass slide obtained with such a microscope. The new scanning microscope is also capable of determining, in parallel, the real-time binding kinetics of multiple molecular species under aqueous conditions.

Original languageEnglish (US)
Article number013708
JournalReview of Scientific Instruments
Volume79
Issue number1
DOIs
StatePublished - 2008

ASJC Scopus subject areas

  • Instrumentation
  • Physics and Astronomy (miscellaneous)

Fingerprint Dive into the research topics of 'A novel high-throughput scanning microscope for label-free detection of protein and small-molecule chemical microarrays'. Together they form a unique fingerprint.

  • Cite this