A novel function for the Caenorhabditis elegans torsin OOC-5 in nucleoporin localization and nuclear import

Michael J W VanGompel, Ken C Q Nguyen, David H. Hall, William T. Dauer, Lesilee S. Rose

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Torsin proteins are AAA+ ATPases that localize to the endoplasmic reticular/nuclear envelope (ER/NE) lumen. A mutation that markedly impairs torsinA function causes the CNS disorder DYT1 dystonia. Abnormalities of NE membranes have been linked to torsinA loss of function and the pathogenesis of DYT1 dystonia, leading us to investigate the role of the Caenorhabditis elegans torsinA homologue OOC-5 at the NE. We report a novel role for torsin in nuclear pore biology. In ooc-5 - mutant germ cell nuclei, nucleoporins (Nups) were mislocalized in large plaques beginning at meiotic entry and persisted throughout meiosis. Moreover, the KASH protein ZYG-12 was mislocalized in ooc-5 gonads. Nups were mislocalized in adult intestinal nuclei and in embryos from mutant mothers. EM analysis revealed vesicle-like structures in the perinuclear space of intestinal and germ cell nuclei, similar to defects reported in torsin-mutant flies and mice. Consistent with a functional disruption of Nups, ooc-5 - mutant embryos displayed impaired nuclear import kinetics, although the nuclear pore-size exclusion barrier was maintained. Our data are the first to demonstrate a requirement for a torsin for normal Nup localization and function and suggest that these functions are likely conserved.

Original languageEnglish (US)
Pages (from-to)1752-1763
Number of pages12
JournalMolecular Biology of the Cell
Volume26
Issue number9
StatePublished - May 1 2015

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Nuclear Pore Complex Proteins
Cell Nucleus Active Transport
Caenorhabditis elegans
Nuclear Pore
Cell Nucleus
Germ Cells
Embryonic Structures
Dystonic Disorders
Dystonia
Nuclear Envelope
Meiosis
Gonads
Diptera
Adenosine Triphosphatases
Proteins
Mutation
Membranes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

VanGompel, M. J. W., Nguyen, K. C. Q., Hall, D. H., Dauer, W. T., & Rose, L. S. (2015). A novel function for the Caenorhabditis elegans torsin OOC-5 in nucleoporin localization and nuclear import. Molecular Biology of the Cell, 26(9), 1752-1763.

A novel function for the Caenorhabditis elegans torsin OOC-5 in nucleoporin localization and nuclear import. / VanGompel, Michael J W; Nguyen, Ken C Q; Hall, David H.; Dauer, William T.; Rose, Lesilee S.

In: Molecular Biology of the Cell, Vol. 26, No. 9, 01.05.2015, p. 1752-1763.

Research output: Contribution to journalArticle

VanGompel, MJW, Nguyen, KCQ, Hall, DH, Dauer, WT & Rose, LS 2015, 'A novel function for the Caenorhabditis elegans torsin OOC-5 in nucleoporin localization and nuclear import', Molecular Biology of the Cell, vol. 26, no. 9, pp. 1752-1763.
VanGompel, Michael J W ; Nguyen, Ken C Q ; Hall, David H. ; Dauer, William T. ; Rose, Lesilee S. / A novel function for the Caenorhabditis elegans torsin OOC-5 in nucleoporin localization and nuclear import. In: Molecular Biology of the Cell. 2015 ; Vol. 26, No. 9. pp. 1752-1763.
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