A novel CsrA titration mechanism regulates fimbrial gene expression in Salmonella typhimurium

Torsten Sterzenbach, Kim T. Nguyen, Sean Paul Nuccio, Maria G. Winter, Christopher A. Vakulskas, Steven Clegg, Tony Romeo, Andreas J Baumler

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A hierarchical control of fimbrial gene expression limits laboratory grown cultures of Salmonella enterica serovar typhimurium (S. typhimurium) to the production of type I fimbriae encoded by the fimAICDHF operon. Here we show that an unlikely culprit, namely the 5′-untranslated region (5′-UTR) of a messenger (m)RNA, coordinated the regulation. Binding of CsrA to the 5′-UTR of the pefACDEF transcript was required for expression of plasmid-encoded fimbriae. The 5′-UTR of the fimAICDHF transcript cooperated with two small untranslated RNAs, termed CsrB and CsrC, in antagonizing the activity of the RNA binding protein CsrA. Through this post-transcriptional mechanism, the 5′-UTR of the fimAICDHF transcript prevented production of PefA, the major structural subunit of plasmid-encoded fimbriae. This regulatory mechanism limits the costly expression of plasmid-encoded fimbriae to host environments in a mouse model. Collectively, our data suggest that the 5′-UTR of an mRNA coordinates a hierarchical control of fimbrial gene expression in S. typhimurium.

Original languageEnglish (US)
Pages (from-to)2872-2883
Number of pages12
JournalEMBO Journal
Volume32
Issue number21
DOIs
StatePublished - Oct 30 2013

Fingerprint

Salmonella
5' Untranslated Regions
Salmonella typhimurium
Titration
Gene expression
Gene Expression
Plasmids
Small Untranslated RNA
Messenger RNA
Salmonella enterica
RNA-Binding Proteins
Operon
RNA

Keywords

  • fimbriae
  • gene regulation
  • Salmonella

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

Cite this

Sterzenbach, T., Nguyen, K. T., Nuccio, S. P., Winter, M. G., Vakulskas, C. A., Clegg, S., ... Baumler, A. J. (2013). A novel CsrA titration mechanism regulates fimbrial gene expression in Salmonella typhimurium. EMBO Journal, 32(21), 2872-2883. https://doi.org/10.1038/emboj.2013.206

A novel CsrA titration mechanism regulates fimbrial gene expression in Salmonella typhimurium. / Sterzenbach, Torsten; Nguyen, Kim T.; Nuccio, Sean Paul; Winter, Maria G.; Vakulskas, Christopher A.; Clegg, Steven; Romeo, Tony; Baumler, Andreas J.

In: EMBO Journal, Vol. 32, No. 21, 30.10.2013, p. 2872-2883.

Research output: Contribution to journalArticle

Sterzenbach, T, Nguyen, KT, Nuccio, SP, Winter, MG, Vakulskas, CA, Clegg, S, Romeo, T & Baumler, AJ 2013, 'A novel CsrA titration mechanism regulates fimbrial gene expression in Salmonella typhimurium', EMBO Journal, vol. 32, no. 21, pp. 2872-2883. https://doi.org/10.1038/emboj.2013.206
Sterzenbach T, Nguyen KT, Nuccio SP, Winter MG, Vakulskas CA, Clegg S et al. A novel CsrA titration mechanism regulates fimbrial gene expression in Salmonella typhimurium. EMBO Journal. 2013 Oct 30;32(21):2872-2883. https://doi.org/10.1038/emboj.2013.206
Sterzenbach, Torsten ; Nguyen, Kim T. ; Nuccio, Sean Paul ; Winter, Maria G. ; Vakulskas, Christopher A. ; Clegg, Steven ; Romeo, Tony ; Baumler, Andreas J. / A novel CsrA titration mechanism regulates fimbrial gene expression in Salmonella typhimurium. In: EMBO Journal. 2013 ; Vol. 32, No. 21. pp. 2872-2883.
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