A novel clinically relevant animal model for studying galectin-3 and its ligands during colon carcinogenesis

Marcelo Hill, Daniel Mazal, Verónica Andrea Biron, Laura Pereira, Luis Ubillos, Edgardo Berriel, Hafiz Ahmed, Teresa Freire, Mariella Rondán, Gerardo R. Vasta, Fu-Tong Liu, María Mercedes Iglesias, Eduardo Osinaga

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Galectin-3 (Gal-3) is a multifunctional protein that plays different roles in cancer biology. To better understand the role of Gal-3 and its ligands during colon carcinogenesis, we studied its expression in tumors induced in rats treated with 1,2-dimethylhydrazine (DMH) and in human tissues. Normal colon from untreated rats showed no staining using two specific monoclonal antibodies. In contrast, morphologically normal colon from DMH-treated rats and dysplastic aberrant crypt foci were strongly stained, indicating that increased Gal-3 expression is an early event during the neoplastic transformation in colon cells. Gal-3 was weakly expressed in adenocarcinomas. Overall, the Gal-3 expression pattern observed in the DMH rat model closely resembles that displayed by human colon stained with the same antibodies. We also found that Gal-3 phosphorylation diminishes in serines while increasing in tyrosines during rat colon carcinogenesis. Finally, we showed that Gal-3-ligands expression is strikingly similar in rat and human malignant colon and in non-malignant tissues. In conclusion, the DMH-induced rat colon cancer model displays expression patterns of Gal-3 and its ligands very similar to those observed in human samples. This animal model should contribute to clarifying the role of Gal-3 in colon carcinogenesis and also to finding effective preventive cancer agents based on Gal-3 targeting.

Original languageEnglish (US)
Pages (from-to)553-565
Number of pages13
JournalJournal of Histochemistry and Cytochemistry
Volume58
Issue number6
DOIs
StatePublished - Jun 2010

Fingerprint

Galectin 3
Colon
Carcinogenesis
Animal Models
Ligands
1,2-Dimethylhydrazine
Aberrant Crypt Foci
Neoplasms
Colonic Neoplasms
Serine
Tyrosine
Adenocarcinoma
Monoclonal Antibodies
Phosphorylation
Staining and Labeling

Keywords

  • Animal models
  • Carcinogenesis
  • Colon cancer
  • Galectin-3

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

A novel clinically relevant animal model for studying galectin-3 and its ligands during colon carcinogenesis. / Hill, Marcelo; Mazal, Daniel; Biron, Verónica Andrea; Pereira, Laura; Ubillos, Luis; Berriel, Edgardo; Ahmed, Hafiz; Freire, Teresa; Rondán, Mariella; Vasta, Gerardo R.; Liu, Fu-Tong; Iglesias, María Mercedes; Osinaga, Eduardo.

In: Journal of Histochemistry and Cytochemistry, Vol. 58, No. 6, 06.2010, p. 553-565.

Research output: Contribution to journalArticle

Hill, M, Mazal, D, Biron, VA, Pereira, L, Ubillos, L, Berriel, E, Ahmed, H, Freire, T, Rondán, M, Vasta, GR, Liu, F-T, Iglesias, MM & Osinaga, E 2010, 'A novel clinically relevant animal model for studying galectin-3 and its ligands during colon carcinogenesis', Journal of Histochemistry and Cytochemistry, vol. 58, no. 6, pp. 553-565. https://doi.org/10.1369/jhc.2010.955237
Hill, Marcelo ; Mazal, Daniel ; Biron, Verónica Andrea ; Pereira, Laura ; Ubillos, Luis ; Berriel, Edgardo ; Ahmed, Hafiz ; Freire, Teresa ; Rondán, Mariella ; Vasta, Gerardo R. ; Liu, Fu-Tong ; Iglesias, María Mercedes ; Osinaga, Eduardo. / A novel clinically relevant animal model for studying galectin-3 and its ligands during colon carcinogenesis. In: Journal of Histochemistry and Cytochemistry. 2010 ; Vol. 58, No. 6. pp. 553-565.
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