A Novel Cellular Model (SPGM 1) of Switching between the pre-B Cell and Myelomonocytic Lineages

Michael Martin, Andreas Strasser, Nicole Baumgarth, Flavia M. Cicuttini, Karen Welch, Evelyn Salvaris, Andrew W. Boyd

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The suspension pro granulocyte/macrophage (SPGM1) cell line was established from a transplantable mouse progranulocytic/promacrophage tumor. Surprisingly, SPGM1 cells expressed a typical CD5 pre-B cell phenotype, being positive for Ly-1 (CD5), PB76, B220 (CD45RA), and the pre-B Ig receptor complex (μH chains, λ5 and vpre-B surrogate L chains, and the IgMα (mb-1) and Igβ (B29) co-receptor molecules). Southern Blot analysis revealed clonal rearrangement of the μH chain locus and germ-line L chain loci. SPGM1 formed blast cell-, macrophage-, and occasional granulocytic colonies in soft agar in the presence of murine IL-3. IL-3 also induced macrophage differentiation of SPGM1 cells in suspension cultures. The earliest changes were detectable at 24 h by Northern blot analysis. IL-3-treatment increased Mac1 mRNA, induced c-fms mRNA, and down-regulated mRNA for μ, λ5, vpre-B and mb-1. After 2 to 4 days the cells were larger, strongly adherent, expressed the macrophage markers Mac1 and F4/80, had lost μH chain and PB76 surface expression, and readily phagocytosed latex beads. Thus SPGM1 has all the characteristic features of a CD5+ pre-B cell line. However, IL-3 predominantly induced SPGM1 to switch its differentiation program from a pre-B cell to a macrophage. This inducible, rapid switch of virtually the entire population provides a unique model for the molecular analysis of mechanisms involved in cell-fate determination.

Original languageEnglish (US)
Pages (from-to)4395-4406
Number of pages12
JournalJournal of Immunology
Volume150
Issue number10
StatePublished - May 15 1993
Externally publishedYes

Fingerprint

B-Lymphoid Precursor Cells
Cell Lineage
Interleukin-3
Macrophages
Messenger RNA
Suspensions
Cell Line
Molecular Models
Southern Blotting
Microspheres
Phagocytosis
Granulocytes
Germ Cells
Northern Blotting
Agar
Immunoglobulin M
Cell Differentiation
Phenotype
Population
Neoplasms

ASJC Scopus subject areas

  • Immunology

Cite this

Martin, M., Strasser, A., Baumgarth, N., Cicuttini, F. M., Welch, K., Salvaris, E., & Boyd, A. W. (1993). A Novel Cellular Model (SPGM 1) of Switching between the pre-B Cell and Myelomonocytic Lineages. Journal of Immunology, 150(10), 4395-4406.

A Novel Cellular Model (SPGM 1) of Switching between the pre-B Cell and Myelomonocytic Lineages. / Martin, Michael; Strasser, Andreas; Baumgarth, Nicole; Cicuttini, Flavia M.; Welch, Karen; Salvaris, Evelyn; Boyd, Andrew W.

In: Journal of Immunology, Vol. 150, No. 10, 15.05.1993, p. 4395-4406.

Research output: Contribution to journalArticle

Martin, M, Strasser, A, Baumgarth, N, Cicuttini, FM, Welch, K, Salvaris, E & Boyd, AW 1993, 'A Novel Cellular Model (SPGM 1) of Switching between the pre-B Cell and Myelomonocytic Lineages', Journal of Immunology, vol. 150, no. 10, pp. 4395-4406.
Martin M, Strasser A, Baumgarth N, Cicuttini FM, Welch K, Salvaris E et al. A Novel Cellular Model (SPGM 1) of Switching between the pre-B Cell and Myelomonocytic Lineages. Journal of Immunology. 1993 May 15;150(10):4395-4406.
Martin, Michael ; Strasser, Andreas ; Baumgarth, Nicole ; Cicuttini, Flavia M. ; Welch, Karen ; Salvaris, Evelyn ; Boyd, Andrew W. / A Novel Cellular Model (SPGM 1) of Switching between the pre-B Cell and Myelomonocytic Lineages. In: Journal of Immunology. 1993 ; Vol. 150, No. 10. pp. 4395-4406.
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