A novel canine lymphoma cell line: A translational and comparative model for lymphoma research

William C. Kisseberth, Murali Vara Prasad Nadella, Matthew Breen, Rachael Thomas, Shannon E. Duke, Sridhar Murahari, Carrie E. Kosarek, William Vernau, Anne C. Avery, Mary Jo Burkhard, Thomas J. Rosol

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40 Scopus citations

Abstract

A novel canine lymphoma cell line, OSW, was established from the malignant pleural effusion of a dog with peripheral T-cell lymphoma. The immunoprofile as determined by flow cytometry was as follows: positive for CD45, CD49d, CD18, CD11a; weakly positive for CD11b, CD11c, CD11d; and negative for CD45RA, CD1a, CD1c, CD3, TCRαβ, TCRγδ, CD4, CD5, CD8a, CD8b, CD90(Thy1), CD21, MHCII, CD14(TUK4), CD34, and MPO. Immunocytochemistry of cytospin preparations was negative for cytoplasmic CD3, CD79a, and MPO, but was positive for CD20. The cell line had an oligoclonal T-cell receptor gamma (TCRγ) gene rearrangement. Array comparative genomic hybridization (aCGH) and single locus probe (SLP) analysis showed that there were copy number increases of loci on dog chromosome 13 (CFA 13), and copy number decreases were evident for regions of CFA 11, 22, 26, 30 and 32, which include several of the more common chromosomal aberrations reported previously in canine lymphoma. The OSW cell line grows rapidly in vitro and is tumorigenic as a xenograft in SCID/NOD mice. OSW represents one of only a few reported canine lymphoma cell lines and is the most thoroughly characterized. This cell line and xenograft represent significant in vitro and in vivo models, respectively, for comparative and translational lymphoma research.

Original languageEnglish (US)
Pages (from-to)1709-1720
Number of pages12
JournalLeukemia Research
Volume31
Issue number12
DOIs
StatePublished - Dec 2007

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Keywords

  • Array comparative genomic hybridization
  • Bioluminescent imaging
  • Canine lymphoma
  • Cell line
  • Xenograft

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Kisseberth, W. C., Nadella, M. V. P., Breen, M., Thomas, R., Duke, S. E., Murahari, S., Kosarek, C. E., Vernau, W., Avery, A. C., Burkhard, M. J., & Rosol, T. J. (2007). A novel canine lymphoma cell line: A translational and comparative model for lymphoma research. Leukemia Research, 31(12), 1709-1720. https://doi.org/10.1016/j.leukres.2007.04.003