A novel canine lymphoma cell line: A translational and comparative model for lymphoma research

William C. Kisseberth, Murali Vara Prasad Nadella, Matthew Breen, Rachael Thomas, Shannon E. Duke, Sridhar Murahari, Carrie E. Kosarek, William Vernau, Anne C. Avery, Mary Jo Burkhard, Thomas J. Rosol

Research output: Contribution to journalArticle

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Abstract

A novel canine lymphoma cell line, OSW, was established from the malignant pleural effusion of a dog with peripheral T-cell lymphoma. The immunoprofile as determined by flow cytometry was as follows: positive for CD45, CD49d, CD18, CD11a; weakly positive for CD11b, CD11c, CD11d; and negative for CD45RA, CD1a, CD1c, CD3, TCRαβ, TCRγδ, CD4, CD5, CD8a, CD8b, CD90(Thy1), CD21, MHCII, CD14(TUK4), CD34, and MPO. Immunocytochemistry of cytospin preparations was negative for cytoplasmic CD3, CD79a, and MPO, but was positive for CD20. The cell line had an oligoclonal T-cell receptor gamma (TCRγ) gene rearrangement. Array comparative genomic hybridization (aCGH) and single locus probe (SLP) analysis showed that there were copy number increases of loci on dog chromosome 13 (CFA 13), and copy number decreases were evident for regions of CFA 11, 22, 26, 30 and 32, which include several of the more common chromosomal aberrations reported previously in canine lymphoma. The OSW cell line grows rapidly in vitro and is tumorigenic as a xenograft in SCID/NOD mice. OSW represents one of only a few reported canine lymphoma cell lines and is the most thoroughly characterized. This cell line and xenograft represent significant in vitro and in vivo models, respectively, for comparative and translational lymphoma research.

Original languageEnglish (US)
Pages (from-to)1709-1720
Number of pages12
JournalLeukemia Research
Volume31
Issue number12
DOIs
StatePublished - Dec 2007

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Canidae
Lymphoma
Cell Line
Research
T-Cell Antigen Receptor
Heterografts
T-Cell Receptor gamma Genes
Dogs
Peripheral T-Cell Lymphoma
Malignant Pleural Effusion
Chromosomes, Human, Pair 13
Inbred NOD Mouse
Translational Medical Research
Comparative Genomic Hybridization
SCID Mice
Gene Rearrangement
Chromosome Aberrations
Flow Cytometry
Immunohistochemistry
In Vitro Techniques

Keywords

  • Array comparative genomic hybridization
  • Bioluminescent imaging
  • Canine lymphoma
  • Cell line
  • Xenograft

ASJC Scopus subject areas

  • Cancer Research
  • Hematology
  • Oncology

Cite this

Kisseberth, W. C., Nadella, M. V. P., Breen, M., Thomas, R., Duke, S. E., Murahari, S., ... Rosol, T. J. (2007). A novel canine lymphoma cell line: A translational and comparative model for lymphoma research. Leukemia Research, 31(12), 1709-1720. https://doi.org/10.1016/j.leukres.2007.04.003

A novel canine lymphoma cell line : A translational and comparative model for lymphoma research. / Kisseberth, William C.; Nadella, Murali Vara Prasad; Breen, Matthew; Thomas, Rachael; Duke, Shannon E.; Murahari, Sridhar; Kosarek, Carrie E.; Vernau, William; Avery, Anne C.; Burkhard, Mary Jo; Rosol, Thomas J.

In: Leukemia Research, Vol. 31, No. 12, 12.2007, p. 1709-1720.

Research output: Contribution to journalArticle

Kisseberth, WC, Nadella, MVP, Breen, M, Thomas, R, Duke, SE, Murahari, S, Kosarek, CE, Vernau, W, Avery, AC, Burkhard, MJ & Rosol, TJ 2007, 'A novel canine lymphoma cell line: A translational and comparative model for lymphoma research', Leukemia Research, vol. 31, no. 12, pp. 1709-1720. https://doi.org/10.1016/j.leukres.2007.04.003
Kisseberth WC, Nadella MVP, Breen M, Thomas R, Duke SE, Murahari S et al. A novel canine lymphoma cell line: A translational and comparative model for lymphoma research. Leukemia Research. 2007 Dec;31(12):1709-1720. https://doi.org/10.1016/j.leukres.2007.04.003
Kisseberth, William C. ; Nadella, Murali Vara Prasad ; Breen, Matthew ; Thomas, Rachael ; Duke, Shannon E. ; Murahari, Sridhar ; Kosarek, Carrie E. ; Vernau, William ; Avery, Anne C. ; Burkhard, Mary Jo ; Rosol, Thomas J. / A novel canine lymphoma cell line : A translational and comparative model for lymphoma research. In: Leukemia Research. 2007 ; Vol. 31, No. 12. pp. 1709-1720.
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