Abstract
A novel canine lymphoma cell line, OSW, was established from the malignant pleural effusion of a dog with peripheral T-cell lymphoma. The immunoprofile as determined by flow cytometry was as follows: positive for CD45, CD49d, CD18, CD11a; weakly positive for CD11b, CD11c, CD11d; and negative for CD45RA, CD1a, CD1c, CD3, TCRαβ, TCRγδ, CD4, CD5, CD8a, CD8b, CD90(Thy1), CD21, MHCII, CD14(TUK4), CD34, and MPO. Immunocytochemistry of cytospin preparations was negative for cytoplasmic CD3, CD79a, and MPO, but was positive for CD20. The cell line had an oligoclonal T-cell receptor gamma (TCRγ) gene rearrangement. Array comparative genomic hybridization (aCGH) and single locus probe (SLP) analysis showed that there were copy number increases of loci on dog chromosome 13 (CFA 13), and copy number decreases were evident for regions of CFA 11, 22, 26, 30 and 32, which include several of the more common chromosomal aberrations reported previously in canine lymphoma. The OSW cell line grows rapidly in vitro and is tumorigenic as a xenograft in SCID/NOD mice. OSW represents one of only a few reported canine lymphoma cell lines and is the most thoroughly characterized. This cell line and xenograft represent significant in vitro and in vivo models, respectively, for comparative and translational lymphoma research.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1709-1720 |
| Number of pages | 12 |
| Journal | Leukemia Research |
| Volume | 31 |
| Issue number | 12 |
| DOIs | |
| State | Published - Dec 2007 |
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Keywords
- Array comparative genomic hybridization
- Bioluminescent imaging
- Canine lymphoma
- Cell line
- Xenograft
ASJC Scopus subject areas
- Cancer Research
- Hematology
- Oncology
Cite this
A novel canine lymphoma cell line : A translational and comparative model for lymphoma research. / Kisseberth, William C.; Nadella, Murali Vara Prasad; Breen, Matthew; Thomas, Rachael; Duke, Shannon E.; Murahari, Sridhar; Kosarek, Carrie E.; Vernau, William; Avery, Anne C.; Burkhard, Mary Jo; Rosol, Thomas J.
In: Leukemia Research, Vol. 31, No. 12, 12.2007, p. 1709-1720.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A novel canine lymphoma cell line
T2 - A translational and comparative model for lymphoma research
AU - Kisseberth, William C.
AU - Nadella, Murali Vara Prasad
AU - Breen, Matthew
AU - Thomas, Rachael
AU - Duke, Shannon E.
AU - Murahari, Sridhar
AU - Kosarek, Carrie E.
AU - Vernau, William
AU - Avery, Anne C.
AU - Burkhard, Mary Jo
AU - Rosol, Thomas J.
PY - 2007/12
Y1 - 2007/12
N2 - A novel canine lymphoma cell line, OSW, was established from the malignant pleural effusion of a dog with peripheral T-cell lymphoma. The immunoprofile as determined by flow cytometry was as follows: positive for CD45, CD49d, CD18, CD11a; weakly positive for CD11b, CD11c, CD11d; and negative for CD45RA, CD1a, CD1c, CD3, TCRαβ, TCRγδ, CD4, CD5, CD8a, CD8b, CD90(Thy1), CD21, MHCII, CD14(TUK4), CD34, and MPO. Immunocytochemistry of cytospin preparations was negative for cytoplasmic CD3, CD79a, and MPO, but was positive for CD20. The cell line had an oligoclonal T-cell receptor gamma (TCRγ) gene rearrangement. Array comparative genomic hybridization (aCGH) and single locus probe (SLP) analysis showed that there were copy number increases of loci on dog chromosome 13 (CFA 13), and copy number decreases were evident for regions of CFA 11, 22, 26, 30 and 32, which include several of the more common chromosomal aberrations reported previously in canine lymphoma. The OSW cell line grows rapidly in vitro and is tumorigenic as a xenograft in SCID/NOD mice. OSW represents one of only a few reported canine lymphoma cell lines and is the most thoroughly characterized. This cell line and xenograft represent significant in vitro and in vivo models, respectively, for comparative and translational lymphoma research.
AB - A novel canine lymphoma cell line, OSW, was established from the malignant pleural effusion of a dog with peripheral T-cell lymphoma. The immunoprofile as determined by flow cytometry was as follows: positive for CD45, CD49d, CD18, CD11a; weakly positive for CD11b, CD11c, CD11d; and negative for CD45RA, CD1a, CD1c, CD3, TCRαβ, TCRγδ, CD4, CD5, CD8a, CD8b, CD90(Thy1), CD21, MHCII, CD14(TUK4), CD34, and MPO. Immunocytochemistry of cytospin preparations was negative for cytoplasmic CD3, CD79a, and MPO, but was positive for CD20. The cell line had an oligoclonal T-cell receptor gamma (TCRγ) gene rearrangement. Array comparative genomic hybridization (aCGH) and single locus probe (SLP) analysis showed that there were copy number increases of loci on dog chromosome 13 (CFA 13), and copy number decreases were evident for regions of CFA 11, 22, 26, 30 and 32, which include several of the more common chromosomal aberrations reported previously in canine lymphoma. The OSW cell line grows rapidly in vitro and is tumorigenic as a xenograft in SCID/NOD mice. OSW represents one of only a few reported canine lymphoma cell lines and is the most thoroughly characterized. This cell line and xenograft represent significant in vitro and in vivo models, respectively, for comparative and translational lymphoma research.
KW - Array comparative genomic hybridization
KW - Bioluminescent imaging
KW - Canine lymphoma
KW - Cell line
KW - Xenograft
UR - http://www.scopus.com/inward/record.url?scp=35748945762&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=35748945762&partnerID=8YFLogxK
U2 - 10.1016/j.leukres.2007.04.003
DO - 10.1016/j.leukres.2007.04.003
M3 - Article
C2 - 17532464
AN - SCOPUS:35748945762
VL - 31
SP - 1709
EP - 1720
JO - Leukemia Research
JF - Leukemia Research
SN - 0145-2126
IS - 12
ER -