A novel bocavirus in canine liver

Linlin Li, Patricia Pesavento, Christian M. Leutenegger, Marko Estrada, Lark L Schneider, Samia N. Naccache, Erik Samayoa, Charles Chiu, Jianming Qiu, Chunlin Wang, Xutao Deng, Eric Delwart

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Background: Bocaviruses are classified as a genus within the Parvoviridae family of single-stranded DNA viruses and are pathogenic in some mammalian species. Two species have been previously reported in dogs, minute virus of canines (MVC), associated with neonatal diseases and fertility disorders; and Canine bocavirus (CBoV), associated with respiratory disease. Findings. In this study using deep sequencing of enriched viral particles from the liver of a dog with severe hemorrhagic gastroenteritis, necrotizing vasculitis, granulomatous lymphadenitis and anuric renal failure, we identified and characterized a novel bocavirus we named Canine bocavirus 3 (CnBoV3). The three major ORFs of CnBoV3 (NS1, NP1 and VP1) shared less than 60% aa identity with those of other bocaviruses qualifying it as a novel species based on ICTV criteria. Inverse PCR showed the presence of concatemerized or circular forms of the genome in liver. Conclusions: We genetically characterized a bocavirus in a dog liver that is highly distinct from prior canine bocaviruses found in respiratory and fecal samples. Its role in this animal's complex disease remains to be determined.

Original languageEnglish (US)
Article number54
JournalVirology Journal
Volume10
DOIs
StatePublished - 2013

Fingerprint

Bocavirus
Canidae
Liver
Dogs
Infant, Newborn, Diseases
Parvoviridae
High-Throughput Nucleotide Sequencing
Lymphadenitis
Animal Diseases
DNA Viruses
Single-Stranded DNA
Gastroenteritis
Vasculitis
Virion
Open Reading Frames
Renal Insufficiency
Fertility

Keywords

  • Canine bocavirus 3
  • Coinfection
  • Episome

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Li, L., Pesavento, P., Leutenegger, C. M., Estrada, M., Schneider, L. L., Naccache, S. N., ... Delwart, E. (2013). A novel bocavirus in canine liver. Virology Journal, 10, [54]. https://doi.org/10.1186/1743-422X-10-54

A novel bocavirus in canine liver. / Li, Linlin; Pesavento, Patricia; Leutenegger, Christian M.; Estrada, Marko; Schneider, Lark L; Naccache, Samia N.; Samayoa, Erik; Chiu, Charles; Qiu, Jianming; Wang, Chunlin; Deng, Xutao; Delwart, Eric.

In: Virology Journal, Vol. 10, 54, 2013.

Research output: Contribution to journalArticle

Li, L, Pesavento, P, Leutenegger, CM, Estrada, M, Schneider, LL, Naccache, SN, Samayoa, E, Chiu, C, Qiu, J, Wang, C, Deng, X & Delwart, E 2013, 'A novel bocavirus in canine liver', Virology Journal, vol. 10, 54. https://doi.org/10.1186/1743-422X-10-54
Li, Linlin ; Pesavento, Patricia ; Leutenegger, Christian M. ; Estrada, Marko ; Schneider, Lark L ; Naccache, Samia N. ; Samayoa, Erik ; Chiu, Charles ; Qiu, Jianming ; Wang, Chunlin ; Deng, Xutao ; Delwart, Eric. / A novel bocavirus in canine liver. In: Virology Journal. 2013 ; Vol. 10.
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