A novel Arabidopsis acetyltransferase interacts with the geminivirus movement protein NSP

Roisin C. McGarry, Yoshimi D. Barron, Miguel F. Carvalho, Janet E. Hill, Daniel Gold, Edwin Cheung, W. Lee Kraus, Sondra G. Lazarowitz

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Protein acetylation is important in regulating DNA-templated processes specifically and protein-protein interactions more generally in eukaryotes. The geminivirus movement protein NSP is essential for virus movement, shuttling the viral DNA genome between the nucleus and the cytoplasm. We have identified a novel Arabidopsis protein, AtNSI, that interacts with NSP. AtNSI is highly conserved among widely divergent plants. Biochemical studies show that its interaction with NSP is direct and that AtNSI acetylates histones, but not NSP, in vitro. Rather, AtNSI specifically acetylates the viral coat protein. AtNSI is a nuclear protein but does not act as a transcriptional coactivator in vitro, which distinguishes it from known eukaryotic histone acetyltransferases. Its overexpression enhances the efficiency of infection by Cabbage leaf curl virus. These findings suggest a role for protein acetylation in coordinating replication of the viral DNA genome with its export from the nucleus.

Original languageEnglish (US)
Pages (from-to)1605-1618
Number of pages14
JournalPlant Cell
Volume15
Issue number7
DOIs
StatePublished - Jul 1 2003
Externally publishedYes

Fingerprint

Geminiviridae
acetyltransferases
Acetyltransferases
Arabidopsis
acetylation
Viral Genome
Viral DNA
Acetylation
Proteins
DNA
proteins
genome
viral proteins
nuclear proteins
Viruses
protein-protein interactions
Histone Acetyltransferases
virus replication
coat proteins
histones

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

Cite this

McGarry, R. C., Barron, Y. D., Carvalho, M. F., Hill, J. E., Gold, D., Cheung, E., ... Lazarowitz, S. G. (2003). A novel Arabidopsis acetyltransferase interacts with the geminivirus movement protein NSP. Plant Cell, 15(7), 1605-1618. https://doi.org/10.1105/tpc.012120

A novel Arabidopsis acetyltransferase interacts with the geminivirus movement protein NSP. / McGarry, Roisin C.; Barron, Yoshimi D.; Carvalho, Miguel F.; Hill, Janet E.; Gold, Daniel; Cheung, Edwin; Kraus, W. Lee; Lazarowitz, Sondra G.

In: Plant Cell, Vol. 15, No. 7, 01.07.2003, p. 1605-1618.

Research output: Contribution to journalArticle

McGarry, RC, Barron, YD, Carvalho, MF, Hill, JE, Gold, D, Cheung, E, Kraus, WL & Lazarowitz, SG 2003, 'A novel Arabidopsis acetyltransferase interacts with the geminivirus movement protein NSP', Plant Cell, vol. 15, no. 7, pp. 1605-1618. https://doi.org/10.1105/tpc.012120
McGarry, Roisin C. ; Barron, Yoshimi D. ; Carvalho, Miguel F. ; Hill, Janet E. ; Gold, Daniel ; Cheung, Edwin ; Kraus, W. Lee ; Lazarowitz, Sondra G. / A novel Arabidopsis acetyltransferase interacts with the geminivirus movement protein NSP. In: Plant Cell. 2003 ; Vol. 15, No. 7. pp. 1605-1618.
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