A novel approach to identifying and quantifying neutrophil extracellular trap formation in septic dogs using immunofluorescence microscopy

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Background: Canine neutrophils release neutrophil extracellular traps (NETs) in response to lipopolysaccharide but NETs from clinical septic dogs had not been identified. The primary aim is to describe the methodology of identifying and quantifying neutrophil extracellular traps (NETs) in cytology samples of septic foci in dogs with sepsis using immunofluorescence microscopy. Cytology samples including endotracheal tracheal wash (ETW), bronchoalveolar lavage (BAL), abdominal and pleural effusion collected from 5 dogs (3 septic, 2 non-septic) were fixed, permeabilized and stained for myeloperoxidase (MPO), citrullinated histone H3 (citH3) and cell-free DNA (cfDNA). Fluorescence microscopy was used to identify and quantify NETs in 10 random views at 40× magnification. NETs were identified based on co-localization of MPO, citH3 and cfDNA. NETs were quantified as a ratio (number of NETs: number of neutrophils). Neutrophils were identified based on cytoplasmic MPO, cellular diameter and nuclear morphology. Results: NETs were identified and quantified in all cytology samples collected from septic dogs. A small number of NETs was documented in one dog with sterile chronic bronchitis. No NETs were found in sterile abdominal effusion collected from one dog with congestive heart failure. Conclusions: Immunofluorescence microscopy could be a useful tool for the study of NETs in dogs with clinical sepsis.

Original languageEnglish (US)
Article number210
JournalBMC Veterinary Research
Volume14
Issue number1
DOIs
StatePublished - Jun 27 2018

Keywords

  • Cell-free DNA
  • Citrullinated histones
  • Peptidylarginine deiminase
  • Sepsis

ASJC Scopus subject areas

  • veterinary(all)

Fingerprint Dive into the research topics of 'A novel approach to identifying and quantifying neutrophil extracellular trap formation in septic dogs using immunofluorescence microscopy'. Together they form a unique fingerprint.

  • Cite this