A novel allele of Saccharomyces cerevisiae NDC1 reveals a potential role for the spindle pole body component Ndc1p in nuclear pore assembly

Corine K. Lau, Thomas H. Giddings, Mark Winey

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Both the spindle pole body (SPB) and the nuclear pore complex (NPC) are essential organelles embedded in the nuclear envelope throughout the life cycle of the budding yeast Saccharomyces cerevisiae. However, the mechanism by which these two multisubunit structures are inserted into the nuclear envelope during their biogenesis is not well understood. We have previously shown that Ndc1p is the only known integral membrane protein that localizes to both the SPBs and the NPCs and is required for SPB duplication. For this study, we generated a novel temperature-sensitive (ts) allele of NDC1 to investigate the role of Ndc1p at the NPCs. Yeast cells carrying this allele (ndc1-39) failed to insert the SPB into the nuclear envelope at the restrictive temperature. Importantly, the double mutation of ndc1-39 and NPC assembly mutant nic96-1 resulted in cells with enhanced growth defects. While nuclear protein import and NPC distribution in the nuclear envelope were unaffected, ndc1-39 mutants failed to properly incorporate the nucleoporin Nup49p into NPCs. These results provide evidence that Ndc1p is required for NPC assembly in addition to its role in SPB duplication. We postulate that Ndc1p is crucial for the biogenesis of both the SPBs and the NPCs at the step of insertion into the nuclear envelope.

Original languageEnglish (US)
Pages (from-to)447-458
Number of pages12
JournalEukaryotic Cell
Volume3
Issue number2
DOIs
StatePublished - Apr 1 2004
Externally publishedYes

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Spindle Pole Bodies
spindle pole body
nucleoporins
Nuclear Pore
Nuclear Envelope
nuclear membrane
Saccharomyces cerevisiae
Alleles
alleles
Nuclear Pore Complex Proteins
yeasts
mutants
Saccharomycetales
Temperature
Cell Nucleus Active Transport
nuclear proteins
Nuclear Proteins
Life Cycle Stages
membrane proteins
Organelles

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Microbiology

Cite this

A novel allele of Saccharomyces cerevisiae NDC1 reveals a potential role for the spindle pole body component Ndc1p in nuclear pore assembly. / Lau, Corine K.; Giddings, Thomas H.; Winey, Mark.

In: Eukaryotic Cell, Vol. 3, No. 2, 01.04.2004, p. 447-458.

Research output: Contribution to journalArticle

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