A non-invasive method for measurement of gastric emptying in mice: Effects of altering fat content and CCK A receptor blockade

K. L. Whited, W. J. Hornof, T. Garcia, D. C. Bohan, R. F. Larson, Helen E Raybould

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

The ability to make repetitive non-invasive measurements of gastric emptying of nutritive solids in awake, unstressed mice is highly desirable. The aim of the present study was to develop such a technique using nuclear scintigraphy and diets differing in triglyceride content. Awakemice were accustomed to light restraint and to feeding cooked, egg white (0.00 g fat g-1), whole egg (0.10 g fat g-1), or egg yolk (0.31 g fat g-1). Gastric emptying of each diet was measured by labelling the test meals with Technetium 99m Mebrofenin and using a conventional gamma camera equipped with a high resolution, parallel hole collimator. Gastric emptying of cooked whole egg was also determined following administration of either vehicle or CCK A receptor antagonist, devazepide. The half-emptying time (t1/2) significantly increased with increasing triglyceride content from 14 ± 5 min to 51 ± 6 min and 82 ± 4 min for egg white, whole egg and egg yolk, respectively. Administration of devazepide significantly decreased t1/2 of whole egg to 28 ± 2 min. These results demonstrate the sensitivity and predictability of this technique in mice and importantly, provide an opportunity to alter the macronutrient or caloric content of the meal to determine effects on gastric emptying.

Original languageEnglish (US)
Pages (from-to)421-427
Number of pages7
JournalNeurogastroenterology and Motility
Volume16
Issue number4
DOIs
StatePublished - Sep 2004

    Fingerprint

Keywords

  • Devazepide
  • Nuclear scintigraphy
  • Triglyceride

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology
  • Neuroscience(all)

Cite this