A Non-invasive Liquid Biopsy Screening of Urine-Derived Exosomes for miRNAs as Biomarkers in Endometrial Cancer Patients

Akhil Srivastava, Katherine Moxley, Rachel Ruskin, Danny Natarajan Dhanasekaran, Yan Daniel Zhao, Rajagopal Ramesh

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Exosomes have great potential to serve as a source of diagnostic and prognostic biomarkers for endometrial cancer (EC). Urine-derived exosomes from patients with EC and patients with symptoms of EC, but without established EC, were used to evaluate a unique miRNA expression profile. Of the 84 miRNA studied, 57 were amplified in qPCR, suggesting the differential packaging of miRNA in exosomes. Further, hsa-miR-200c-3p was identified to be enriched the most. Various bioinformatics and in silico tools were used to evaluate the biological significance of hsa-miR-200c-3p in EC. We conclude that differential miRNA in exosomes can be utilized for discovery of biomarker signatures and EC diagnosis; hsa-miR-200c-3p is one such candidate. Urine-derived exosomes pave the way for the development of non-invasive biomarkers.

Original languageEnglish (US)
Article number82
JournalAAPS Journal
Volume20
Issue number5
DOIs
StatePublished - Sep 1 2018
Externally publishedYes

Keywords

  • biomarkers
  • endometrial cancer
  • exosomes
  • liquid biopsy
  • miRNA

ASJC Scopus subject areas

  • Pharmaceutical Science

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