A non-functional arginine biosynthetic pathway in polyoma infected-mouse embryo cells

A. L. Winters, R. A. Consigli, Quinton Rogers

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The normal pathway for the biosynthesis of arginine was non-functional in polyoma infected-mouse cells. During viral replication, equimolar concentration of citrulline substituted for arginine but equimolar or ten times equimolar concentration of ornithine did not replace arginine. Purified polyoma virus obtained from infected cultures that were allowed to incorporate citrulline-14C revealed upon amino acid analysis that the label was found exclusively as arginine-14C. When the experiment was repeated using ornithine-14C the label was found as proline-14C and glutamate-14C and not arginine. Both normal and polyoma infected-mouse embryo cells possessed an active ornithine carbamoyltransferase. The inability of ornithine to serve as an arginine precursor appears to be due to the fact that exogenous ornithine is utilized by ornithine-δ-transaminase to synthesize proline and glutamate and not by ornithine carbamoyltransferase to synthesize citrulline.

Original languageEnglish (US)
Pages (from-to)1044-1050
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume47
Issue number5
DOIs
StatePublished - Jun 9 1972

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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