A new robust diagnostic polymerase chain reaction for determining the mating status of female Anopheles gambiae mosquitoes

Kija R. Ng'habi, Ashley Horton, Bart G J Knols, Gregory C Lanzaro

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The principal malaria vector in Africa, Anopheles gambiae, contains two pairs of autosomes and one pair of sex chromosomes. The Y chromosome is only associated with males and other Y chromosome-specific DNA sequences, which are transferred to women during mating. A reliable tool to determine the mating status of dried wild An. gambiae females is currently lacking. DNA was extracted from dried virgin and mated females and used to test whether Y chromosome-specific polymerase chain reaction (PCR) markers can be successfully amplified and used as a predictor of mating. Here we report a new PCR-based method to determine the mating status among successfully inseminated and virgin wild An. gambiae females, using three male-specific primers. This dissection-free method has the potential to facilitate studies of both population demographics and gene flow from dried mosquito samples routinely collected in epidemiologic monitoring and aid existing and new malaria-vector control approaches.

Original languageEnglish (US)
Pages (from-to)485-487
Number of pages3
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume77
Issue number3
StatePublished - Sep 2007

Fingerprint

Anopheles gambiae
Y Chromosome
Culicidae
Polymerase Chain Reaction
Malaria
Epidemiological Monitoring
Sex Chromosomes
Gene Flow
Dissection
Demography
DNA
Population

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

Cite this

A new robust diagnostic polymerase chain reaction for determining the mating status of female Anopheles gambiae mosquitoes. / Ng'habi, Kija R.; Horton, Ashley; Knols, Bart G J; Lanzaro, Gregory C.

In: American Journal of Tropical Medicine and Hygiene, Vol. 77, No. 3, 09.2007, p. 485-487.

Research output: Contribution to journalArticle

@article{64cdb0edf5d446fb916eef7d64201810,
title = "A new robust diagnostic polymerase chain reaction for determining the mating status of female Anopheles gambiae mosquitoes",
abstract = "The principal malaria vector in Africa, Anopheles gambiae, contains two pairs of autosomes and one pair of sex chromosomes. The Y chromosome is only associated with males and other Y chromosome-specific DNA sequences, which are transferred to women during mating. A reliable tool to determine the mating status of dried wild An. gambiae females is currently lacking. DNA was extracted from dried virgin and mated females and used to test whether Y chromosome-specific polymerase chain reaction (PCR) markers can be successfully amplified and used as a predictor of mating. Here we report a new PCR-based method to determine the mating status among successfully inseminated and virgin wild An. gambiae females, using three male-specific primers. This dissection-free method has the potential to facilitate studies of both population demographics and gene flow from dried mosquito samples routinely collected in epidemiologic monitoring and aid existing and new malaria-vector control approaches.",
author = "Ng'habi, {Kija R.} and Ashley Horton and Knols, {Bart G J} and Lanzaro, {Gregory C}",
year = "2007",
month = "9",
language = "English (US)",
volume = "77",
pages = "485--487",
journal = "American Journal of Tropical Medicine and Hygiene",
issn = "0002-9637",
publisher = "American Society of Tropical Medicine and Hygiene",
number = "3",

}

TY - JOUR

T1 - A new robust diagnostic polymerase chain reaction for determining the mating status of female Anopheles gambiae mosquitoes

AU - Ng'habi, Kija R.

AU - Horton, Ashley

AU - Knols, Bart G J

AU - Lanzaro, Gregory C

PY - 2007/9

Y1 - 2007/9

N2 - The principal malaria vector in Africa, Anopheles gambiae, contains two pairs of autosomes and one pair of sex chromosomes. The Y chromosome is only associated with males and other Y chromosome-specific DNA sequences, which are transferred to women during mating. A reliable tool to determine the mating status of dried wild An. gambiae females is currently lacking. DNA was extracted from dried virgin and mated females and used to test whether Y chromosome-specific polymerase chain reaction (PCR) markers can be successfully amplified and used as a predictor of mating. Here we report a new PCR-based method to determine the mating status among successfully inseminated and virgin wild An. gambiae females, using three male-specific primers. This dissection-free method has the potential to facilitate studies of both population demographics and gene flow from dried mosquito samples routinely collected in epidemiologic monitoring and aid existing and new malaria-vector control approaches.

AB - The principal malaria vector in Africa, Anopheles gambiae, contains two pairs of autosomes and one pair of sex chromosomes. The Y chromosome is only associated with males and other Y chromosome-specific DNA sequences, which are transferred to women during mating. A reliable tool to determine the mating status of dried wild An. gambiae females is currently lacking. DNA was extracted from dried virgin and mated females and used to test whether Y chromosome-specific polymerase chain reaction (PCR) markers can be successfully amplified and used as a predictor of mating. Here we report a new PCR-based method to determine the mating status among successfully inseminated and virgin wild An. gambiae females, using three male-specific primers. This dissection-free method has the potential to facilitate studies of both population demographics and gene flow from dried mosquito samples routinely collected in epidemiologic monitoring and aid existing and new malaria-vector control approaches.

UR - http://www.scopus.com/inward/record.url?scp=35748956854&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=35748956854&partnerID=8YFLogxK

M3 - Article

VL - 77

SP - 485

EP - 487

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

SN - 0002-9637

IS - 3

ER -