A new robust diagnostic polymerase chain reaction for determining the mating status of female Anopheles gambiae mosquitoes

Kija R. Ng'habi, Ashley Horton, Bart G J Knols, Gregory C Lanzaro

Research output: Contribution to journalArticle

8 Scopus citations


The principal malaria vector in Africa, Anopheles gambiae, contains two pairs of autosomes and one pair of sex chromosomes. The Y chromosome is only associated with males and other Y chromosome-specific DNA sequences, which are transferred to women during mating. A reliable tool to determine the mating status of dried wild An. gambiae females is currently lacking. DNA was extracted from dried virgin and mated females and used to test whether Y chromosome-specific polymerase chain reaction (PCR) markers can be successfully amplified and used as a predictor of mating. Here we report a new PCR-based method to determine the mating status among successfully inseminated and virgin wild An. gambiae females, using three male-specific primers. This dissection-free method has the potential to facilitate studies of both population demographics and gene flow from dried mosquito samples routinely collected in epidemiologic monitoring and aid existing and new malaria-vector control approaches.

Original languageEnglish (US)
Pages (from-to)485-487
Number of pages3
JournalAmerican Journal of Tropical Medicine and Hygiene
Issue number3
StatePublished - Sep 2007


ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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