A new protoparvovirus in human fecal samples and cutaneous T cell lymphomas (mycosis fungoides)

Tung G. Phan, Brigitte Dreno, Antonio Charlys da Costa, Linlin Li, Patricia Orlandi, Xutao Deng, Beatrix Kapusinszky, Juliana Siqueira, Anne Chantal Knol, Franck Halary, Jacques Dantal, Kathleen A. Alexander, Patricia Pesavento, Eric Delwart

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We genetically characterized seven nearly complete genomes in the protoparvovirus genus from the feces of children with diarrhea. The viruses, provisionally named cutaviruses (CutaV), varied by 1-6% nucleotides and shared ~76% and ~82% amino acid identity with the NS1 and VP1 of human bufaviruses, their closest relatives. Using PCR, cutavirus DNA was found in 1.6% (4/245) and 1% (1/100) of diarrhea samples from Brazil and Botswana respectively. In silico analysis of pre-existing metagenomics datasets then revealed closely related parvovirus genomes in skin biopsies from patients with epidermotropic cutaneous T-cell lymphoma (CTCL or mycosis fungoides). PCR of skin biopsies yielded cutavirus DNA in 4/17 CTCL, 0/10 skin carcinoma, and 0/21 normal or noncancerous skin biopsies. In situ hybridization of CTCL skin biopsies detected viral genome within rare individual cells in regions of neoplastic infiltrations. The influence of cutavirus infection on human enteric functions and possible oncolytic role in CTCL progression remain to be determined.

Original languageEnglish (US)
Pages (from-to)299-305
Number of pages7
JournalVirology
Volume496
DOIs
StatePublished - Sep 1 2016

Fingerprint

Cutaneous T-Cell Lymphoma
Mycosis Fungoides
Skin
Biopsy
Diarrhea
Genome
Botswana
Metagenomics
Polymerase Chain Reaction
Parvovirus
Viral Genome
DNA
Feces
Computer Simulation
In Situ Hybridization
Brazil
Nucleotides
Viruses
Carcinoma
Amino Acids

Keywords

  • CTCL
  • Cutavirus
  • Oncolytic
  • Protoparvovirus

ASJC Scopus subject areas

  • Medicine(all)
  • Virology

Cite this

Phan, T. G., Dreno, B., da Costa, A. C., Li, L., Orlandi, P., Deng, X., ... Delwart, E. (2016). A new protoparvovirus in human fecal samples and cutaneous T cell lymphomas (mycosis fungoides). Virology, 496, 299-305. https://doi.org/10.1016/j.virol.2016.06.013

A new protoparvovirus in human fecal samples and cutaneous T cell lymphomas (mycosis fungoides). / Phan, Tung G.; Dreno, Brigitte; da Costa, Antonio Charlys; Li, Linlin; Orlandi, Patricia; Deng, Xutao; Kapusinszky, Beatrix; Siqueira, Juliana; Knol, Anne Chantal; Halary, Franck; Dantal, Jacques; Alexander, Kathleen A.; Pesavento, Patricia; Delwart, Eric.

In: Virology, Vol. 496, 01.09.2016, p. 299-305.

Research output: Contribution to journalArticle

Phan, TG, Dreno, B, da Costa, AC, Li, L, Orlandi, P, Deng, X, Kapusinszky, B, Siqueira, J, Knol, AC, Halary, F, Dantal, J, Alexander, KA, Pesavento, P & Delwart, E 2016, 'A new protoparvovirus in human fecal samples and cutaneous T cell lymphomas (mycosis fungoides)', Virology, vol. 496, pp. 299-305. https://doi.org/10.1016/j.virol.2016.06.013
Phan, Tung G. ; Dreno, Brigitte ; da Costa, Antonio Charlys ; Li, Linlin ; Orlandi, Patricia ; Deng, Xutao ; Kapusinszky, Beatrix ; Siqueira, Juliana ; Knol, Anne Chantal ; Halary, Franck ; Dantal, Jacques ; Alexander, Kathleen A. ; Pesavento, Patricia ; Delwart, Eric. / A new protoparvovirus in human fecal samples and cutaneous T cell lymphomas (mycosis fungoides). In: Virology. 2016 ; Vol. 496. pp. 299-305.
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