A new point mutation (P157S) in the reverse transcriptase of human immunodeficiency virus type 1 confers low-level resistance to (-)-β-2',3'- dideoxy-3'-thiacytidine

Robert A. Smith; George J. Klarmann; Kirsten M. Stray; Uta K. Von Schwedler; Raymond F. Schinazi; Bradley D. Preston; Thomas W. North

A new point mutation (P157S) in the reverse transcriptase of human immunodeficiency virus type 1 confers low-level resistance to (-)-β-2',3'- dideoxy-3'-thiacytidine

Robert A. Smith, George J. Klarmann, Kirsten M. Stray, Uta K. Von Schwedler, Raymond F. Schinazi, Bradley D. Preston, Thomas W. North

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

A P157S mutation in the reverse transcriptase (RT) of human immunodeficiency virus type 1 conferred fivefold resistance to (-)-β-2',3'- dideoxy-3'-thiacytidine in cell culture. Interestingly, the P157S mutation resulted in increased sensitivity (two- to threefold) to 3'-azido-3'- deoxythymidine (AZT) and to (R)-9-(2-phosphonyimethoxypropyl)adenine (PMPA). A similar increase in susceptibility to AZT and to PMPA was also conferred by the M184V mutation in RT.

Original language	English (US)
Pages (from-to)	2077-2080
Number of pages	4
Journal	Antimicrobial Agents and Chemotherapy
Volume	43
Issue number	8
State	Published - Aug 1999

ASJC Scopus subject areas

Pharmacology (medical)

Cite this

A new point mutation (P157S) in the reverse transcriptase of human immunodeficiency virus type 1 confers low-level resistance to (-)-β-2',3'- dideoxy-3'-thiacytidine. / Smith, Robert A.; Klarmann, George J.; Stray, Kirsten M.; Von Schwedler, Uta K.; Schinazi, Raymond F.; Preston, Bradley D.; North, Thomas W.

In: Antimicrobial Agents and Chemotherapy, Vol. 43, No. 8, 08.1999, p. 2077-2080.

Research output: Contribution to journal › Article › peer-review

Smith, Robert A. ; Klarmann, George J. ; Stray, Kirsten M. ; Von Schwedler, Uta K. ; Schinazi, Raymond F. ; Preston, Bradley D. ; North, Thomas W. / A new point mutation (P157S) in the reverse transcriptase of human immunodeficiency virus type 1 confers low-level resistance to (-)-β-2',3'- dideoxy-3'-thiacytidine. In: Antimicrobial Agents and Chemotherapy. 1999 ; Vol. 43, No. 8. pp. 2077-2080.

@article{22336bee0a214aef8ba217118e5e0764,

title = "A new point mutation (P157S) in the reverse transcriptase of human immunodeficiency virus type 1 confers low-level resistance to (-)-β-2',3'- dideoxy-3'-thiacytidine",

abstract = "A P157S mutation in the reverse transcriptase (RT) of human immunodeficiency virus type 1 conferred fivefold resistance to (-)-β-2',3'- dideoxy-3'-thiacytidine in cell culture. Interestingly, the P157S mutation resulted in increased sensitivity (two- to threefold) to 3'-azido-3'- deoxythymidine (AZT) and to (R)-9-(2-phosphonyimethoxypropyl)adenine (PMPA). A similar increase in susceptibility to AZT and to PMPA was also conferred by the M184V mutation in RT.",

author = "Smith, {Robert A.} and Klarmann, {George J.} and Stray, {Kirsten M.} and {Von Schwedler}, {Uta K.} and Schinazi, {Raymond F.} and Preston, {Bradley D.} and North, {Thomas W.}",

year = "1999",

month = aug,

language = "English (US)",

volume = "43",

pages = "2077--2080",

journal = "Antimicrobial Agents and Chemotherapy",

issn = "0066-4804",

publisher = "American Society for Microbiology",

number = "8",

}

TY - JOUR

T1 - A new point mutation (P157S) in the reverse transcriptase of human immunodeficiency virus type 1 confers low-level resistance to (-)-β-2',3'- dideoxy-3'-thiacytidine

AU - Smith, Robert A.

AU - Klarmann, George J.

AU - Stray, Kirsten M.

AU - Von Schwedler, Uta K.

AU - Schinazi, Raymond F.

AU - Preston, Bradley D.

AU - North, Thomas W.

PY - 1999/8

Y1 - 1999/8

N2 - A P157S mutation in the reverse transcriptase (RT) of human immunodeficiency virus type 1 conferred fivefold resistance to (-)-β-2',3'- dideoxy-3'-thiacytidine in cell culture. Interestingly, the P157S mutation resulted in increased sensitivity (two- to threefold) to 3'-azido-3'- deoxythymidine (AZT) and to (R)-9-(2-phosphonyimethoxypropyl)adenine (PMPA). A similar increase in susceptibility to AZT and to PMPA was also conferred by the M184V mutation in RT.

AB - A P157S mutation in the reverse transcriptase (RT) of human immunodeficiency virus type 1 conferred fivefold resistance to (-)-β-2',3'- dideoxy-3'-thiacytidine in cell culture. Interestingly, the P157S mutation resulted in increased sensitivity (two- to threefold) to 3'-azido-3'- deoxythymidine (AZT) and to (R)-9-(2-phosphonyimethoxypropyl)adenine (PMPA). A similar increase in susceptibility to AZT and to PMPA was also conferred by the M184V mutation in RT.

UR - http://www.scopus.com/inward/record.url?scp=0032817243&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032817243&partnerID=8YFLogxK

M3 - Article

C2 - 10428942

AN - SCOPUS:0032817243

VL - 43

SP - 2077

EP - 2080

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 8

ER -

A new point mutation (P157S) in the reverse transcriptase of human immunodeficiency virus type 1 confers low-level resistance to (-)-β-2',3'- dideoxy-3'-thiacytidine

Abstract

ASJC Scopus subject areas

Other files and links

Fingerprint

Cite this