A new point mutation (P157S) in the reverse transcriptase of human immunodeficiency virus type 1 confers low-level resistance to (-)-β-2',3'- dideoxy-3'-thiacytidine

Robert A. Smith, George J. Klarmann, Kirsten M. Stray, Uta K. Von Schwedler, Raymond F. Schinazi, Bradley D. Preston, Thomas W. North

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A P157S mutation in the reverse transcriptase (RT) of human immunodeficiency virus type 1 conferred fivefold resistance to (-)-β-2',3'- dideoxy-3'-thiacytidine in cell culture. Interestingly, the P157S mutation resulted in increased sensitivity (two- to threefold) to 3'-azido-3'- deoxythymidine (AZT) and to (R)-9-(2-phosphonyimethoxypropyl)adenine (PMPA). A similar increase in susceptibility to AZT and to PMPA was also conferred by the M184V mutation in RT.

Original languageEnglish (US)
Pages (from-to)2077-2080
Number of pages4
JournalAntimicrobial Agents and Chemotherapy
Volume43
Issue number8
StatePublished - Aug 1999

Fingerprint

Lamivudine
Point Mutation
HIV-1
Adenine
Mutation
Zidovudine
RNA-Directed DNA Polymerase
Cell Culture Techniques
Human immunodeficiency virus 1 reverse transcriptase

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Smith, R. A., Klarmann, G. J., Stray, K. M., Von Schwedler, U. K., Schinazi, R. F., Preston, B. D., & North, T. W. (1999). A new point mutation (P157S) in the reverse transcriptase of human immunodeficiency virus type 1 confers low-level resistance to (-)-β-2',3'- dideoxy-3'-thiacytidine. Antimicrobial Agents and Chemotherapy, 43(8), 2077-2080.

A new point mutation (P157S) in the reverse transcriptase of human immunodeficiency virus type 1 confers low-level resistance to (-)-β-2',3'- dideoxy-3'-thiacytidine. / Smith, Robert A.; Klarmann, George J.; Stray, Kirsten M.; Von Schwedler, Uta K.; Schinazi, Raymond F.; Preston, Bradley D.; North, Thomas W.

In: Antimicrobial Agents and Chemotherapy, Vol. 43, No. 8, 08.1999, p. 2077-2080.

Research output: Contribution to journalArticle

Smith, Robert A. ; Klarmann, George J. ; Stray, Kirsten M. ; Von Schwedler, Uta K. ; Schinazi, Raymond F. ; Preston, Bradley D. ; North, Thomas W. / A new point mutation (P157S) in the reverse transcriptase of human immunodeficiency virus type 1 confers low-level resistance to (-)-β-2',3'- dideoxy-3'-thiacytidine. In: Antimicrobial Agents and Chemotherapy. 1999 ; Vol. 43, No. 8. pp. 2077-2080.
@article{22336bee0a214aef8ba217118e5e0764,
title = "A new point mutation (P157S) in the reverse transcriptase of human immunodeficiency virus type 1 confers low-level resistance to (-)-β-2',3'- dideoxy-3'-thiacytidine",
abstract = "A P157S mutation in the reverse transcriptase (RT) of human immunodeficiency virus type 1 conferred fivefold resistance to (-)-β-2',3'- dideoxy-3'-thiacytidine in cell culture. Interestingly, the P157S mutation resulted in increased sensitivity (two- to threefold) to 3'-azido-3'- deoxythymidine (AZT) and to (R)-9-(2-phosphonyimethoxypropyl)adenine (PMPA). A similar increase in susceptibility to AZT and to PMPA was also conferred by the M184V mutation in RT.",
author = "Smith, {Robert A.} and Klarmann, {George J.} and Stray, {Kirsten M.} and {Von Schwedler}, {Uta K.} and Schinazi, {Raymond F.} and Preston, {Bradley D.} and North, {Thomas W.}",
year = "1999",
month = "8",
language = "English (US)",
volume = "43",
pages = "2077--2080",
journal = "Antimicrobial Agents and Chemotherapy",
issn = "0066-4804",
publisher = "American Society for Microbiology",
number = "8",

}

TY - JOUR

T1 - A new point mutation (P157S) in the reverse transcriptase of human immunodeficiency virus type 1 confers low-level resistance to (-)-β-2',3'- dideoxy-3'-thiacytidine

AU - Smith, Robert A.

AU - Klarmann, George J.

AU - Stray, Kirsten M.

AU - Von Schwedler, Uta K.

AU - Schinazi, Raymond F.

AU - Preston, Bradley D.

AU - North, Thomas W.

PY - 1999/8

Y1 - 1999/8

N2 - A P157S mutation in the reverse transcriptase (RT) of human immunodeficiency virus type 1 conferred fivefold resistance to (-)-β-2',3'- dideoxy-3'-thiacytidine in cell culture. Interestingly, the P157S mutation resulted in increased sensitivity (two- to threefold) to 3'-azido-3'- deoxythymidine (AZT) and to (R)-9-(2-phosphonyimethoxypropyl)adenine (PMPA). A similar increase in susceptibility to AZT and to PMPA was also conferred by the M184V mutation in RT.

AB - A P157S mutation in the reverse transcriptase (RT) of human immunodeficiency virus type 1 conferred fivefold resistance to (-)-β-2',3'- dideoxy-3'-thiacytidine in cell culture. Interestingly, the P157S mutation resulted in increased sensitivity (two- to threefold) to 3'-azido-3'- deoxythymidine (AZT) and to (R)-9-(2-phosphonyimethoxypropyl)adenine (PMPA). A similar increase in susceptibility to AZT and to PMPA was also conferred by the M184V mutation in RT.

UR - http://www.scopus.com/inward/record.url?scp=0032817243&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032817243&partnerID=8YFLogxK

M3 - Article

C2 - 10428942

AN - SCOPUS:0032817243

VL - 43

SP - 2077

EP - 2080

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

SN - 0066-4804

IS - 8

ER -