A new multiplex SNP genotyping assay for detecting hybridization and introgression between the M and S molecular forms of Anopheles gambiae

Yoosook Lee, Clare D. Marsden, Catelyn Nieman, Gregory C Lanzaro

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The M and S forms of Anopheles gambiae have been the subject of intense study, but are morphologically indistinguishable and can only be identified using molecular techniques. PCR-based assays to distinguish the two forms have been designed and applied widely. However, the application of these assays towards identifying hybrids between the two forms, and backcrossed hybrids in particular, has been problematic as the currently available diagnostic assays are based on single locus and/or are located within a multicopy gene. Here, we present an alternative genotyping method for detecting hybridization and introgression between M and S molecular forms based on a multilocus panel of single-nucleotide polymorphisms (SNPs) fixed between the M and S forms. The panel of SNPs employed is located in so-called islands of divergence leading us to describe this method as the 'Divergence Island SNP' (DIS) assay. We show this multilocus SNP genotyping approach can robustly and accurately detect F1 hybrids as well as backcrossed individuals.

Original languageEnglish (US)
Pages (from-to)297-305
Number of pages9
JournalMolecular Ecology Resources
Volume14
Issue number2
DOIs
StatePublished - Mar 2014

Fingerprint

Anopheles gambiae
introgression
genotyping
single nucleotide polymorphism
Single Nucleotide Polymorphism
polymorphism
hybridization
assay
assays
Islands
divergence
methodology
Polymerase Chain Reaction
loci
gene
Genes
genes
method

Keywords

  • Ecological genetics
  • Hybridization
  • Insects
  • Population genetics - empirical
  • Speciation

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Biotechnology
  • Genetics

Cite this

A new multiplex SNP genotyping assay for detecting hybridization and introgression between the M and S molecular forms of Anopheles gambiae. / Lee, Yoosook; Marsden, Clare D.; Nieman, Catelyn; Lanzaro, Gregory C.

In: Molecular Ecology Resources, Vol. 14, No. 2, 03.2014, p. 297-305.

Research output: Contribution to journalArticle

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