A new method for measuring D-dimer using immunoturbidometry: A study of 255 patients with suspected pulmonary embolism and deep vein thrombosis

R. C. Gosselin, John T Owings, Garth H Utter, R. C. Jacoby, E. C. Larkin

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

D-Dimer testing has been suggested as a non-invasive method for the exclusion of pulmonary embolism (PE) and deep vein thrombosis (DVT). In this study, we compared a new method, the Miniquant D-dimer (Biopool International, Ventura, California, USA) to other previously validated D-dimer methods used for the purpose. Patients who were undergoing a definitive diagnostic study for thromboembolism had a blood sample drawn at that time. A whole-blood D-dimer (SimpliRed; Agen Biomedical Ltd, Brisbane, Australia) test was performed, and residual plasma was frozen and later analyzed using two enzyme-linked immunosorbent assay (ELISA) methods (D-dimer Gold; Agen, and Asserachrome D-Di; Stago International, Parsippany, New Jersey, USA) and the Miniquant D-dimer. Once all samples were analyzed, the correlation and accuracy of the Miniquant was compared with the ELISA method using Spearman's regression and Dunn's multiple paired comparison. All D-dimer methods were compared with radiographic studies. The data was analyzed collectively and segregated into in-patient (n = 112) and outpatient (n = 143) populations. The Miniquant D-dimer sensitivity, specificity and negative predictive value (NPV) for all patients were 95, 21, and 94% for DVT, and 100, 26, and 100% for PE. This new D-dimer method demonstrates acceptable sensitivity in patients with PE and DVT and, based on the high NPV of this method, it can be used for the exclusion of thromboembolism.

Original languageEnglish (US)
Pages (from-to)715-721
Number of pages7
JournalBlood Coagulation and Fibrinolysis
Volume11
Issue number8
DOIs
StatePublished - 2000

Fingerprint

Pulmonary Embolism
Venous Thrombosis
Thromboembolism
Enzyme-Linked Immunosorbent Assay
fibrin fragment D
Matched-Pair Analysis
Gold
Outpatients
Sensitivity and Specificity
Population

Keywords

  • D-dimer
  • Deep vein thrombosis
  • Pulmonary embolism

ASJC Scopus subject areas

  • Hematology

Cite this

@article{e7dc37a26b2d42778b3ac4614f7bc605,
title = "A new method for measuring D-dimer using immunoturbidometry: A study of 255 patients with suspected pulmonary embolism and deep vein thrombosis",
abstract = "D-Dimer testing has been suggested as a non-invasive method for the exclusion of pulmonary embolism (PE) and deep vein thrombosis (DVT). In this study, we compared a new method, the Miniquant D-dimer (Biopool International, Ventura, California, USA) to other previously validated D-dimer methods used for the purpose. Patients who were undergoing a definitive diagnostic study for thromboembolism had a blood sample drawn at that time. A whole-blood D-dimer (SimpliRed; Agen Biomedical Ltd, Brisbane, Australia) test was performed, and residual plasma was frozen and later analyzed using two enzyme-linked immunosorbent assay (ELISA) methods (D-dimer Gold; Agen, and Asserachrome D-Di; Stago International, Parsippany, New Jersey, USA) and the Miniquant D-dimer. Once all samples were analyzed, the correlation and accuracy of the Miniquant was compared with the ELISA method using Spearman's regression and Dunn's multiple paired comparison. All D-dimer methods were compared with radiographic studies. The data was analyzed collectively and segregated into in-patient (n = 112) and outpatient (n = 143) populations. The Miniquant D-dimer sensitivity, specificity and negative predictive value (NPV) for all patients were 95, 21, and 94{\%} for DVT, and 100, 26, and 100{\%} for PE. This new D-dimer method demonstrates acceptable sensitivity in patients with PE and DVT and, based on the high NPV of this method, it can be used for the exclusion of thromboembolism.",
keywords = "D-dimer, Deep vein thrombosis, Pulmonary embolism",
author = "Gosselin, {R. C.} and Owings, {John T} and Utter, {Garth H} and Jacoby, {R. C.} and Larkin, {E. C.}",
year = "2000",
doi = "10.1097/00001721-200012000-00004",
language = "English (US)",
volume = "11",
pages = "715--721",
journal = "Blood Coagulation and Fibrinolysis",
issn = "0957-5235",
publisher = "Lippincott Williams and Wilkins",
number = "8",

}

TY - JOUR

T1 - A new method for measuring D-dimer using immunoturbidometry

T2 - A study of 255 patients with suspected pulmonary embolism and deep vein thrombosis

AU - Gosselin, R. C.

AU - Owings, John T

AU - Utter, Garth H

AU - Jacoby, R. C.

AU - Larkin, E. C.

PY - 2000

Y1 - 2000

N2 - D-Dimer testing has been suggested as a non-invasive method for the exclusion of pulmonary embolism (PE) and deep vein thrombosis (DVT). In this study, we compared a new method, the Miniquant D-dimer (Biopool International, Ventura, California, USA) to other previously validated D-dimer methods used for the purpose. Patients who were undergoing a definitive diagnostic study for thromboembolism had a blood sample drawn at that time. A whole-blood D-dimer (SimpliRed; Agen Biomedical Ltd, Brisbane, Australia) test was performed, and residual plasma was frozen and later analyzed using two enzyme-linked immunosorbent assay (ELISA) methods (D-dimer Gold; Agen, and Asserachrome D-Di; Stago International, Parsippany, New Jersey, USA) and the Miniquant D-dimer. Once all samples were analyzed, the correlation and accuracy of the Miniquant was compared with the ELISA method using Spearman's regression and Dunn's multiple paired comparison. All D-dimer methods were compared with radiographic studies. The data was analyzed collectively and segregated into in-patient (n = 112) and outpatient (n = 143) populations. The Miniquant D-dimer sensitivity, specificity and negative predictive value (NPV) for all patients were 95, 21, and 94% for DVT, and 100, 26, and 100% for PE. This new D-dimer method demonstrates acceptable sensitivity in patients with PE and DVT and, based on the high NPV of this method, it can be used for the exclusion of thromboembolism.

AB - D-Dimer testing has been suggested as a non-invasive method for the exclusion of pulmonary embolism (PE) and deep vein thrombosis (DVT). In this study, we compared a new method, the Miniquant D-dimer (Biopool International, Ventura, California, USA) to other previously validated D-dimer methods used for the purpose. Patients who were undergoing a definitive diagnostic study for thromboembolism had a blood sample drawn at that time. A whole-blood D-dimer (SimpliRed; Agen Biomedical Ltd, Brisbane, Australia) test was performed, and residual plasma was frozen and later analyzed using two enzyme-linked immunosorbent assay (ELISA) methods (D-dimer Gold; Agen, and Asserachrome D-Di; Stago International, Parsippany, New Jersey, USA) and the Miniquant D-dimer. Once all samples were analyzed, the correlation and accuracy of the Miniquant was compared with the ELISA method using Spearman's regression and Dunn's multiple paired comparison. All D-dimer methods were compared with radiographic studies. The data was analyzed collectively and segregated into in-patient (n = 112) and outpatient (n = 143) populations. The Miniquant D-dimer sensitivity, specificity and negative predictive value (NPV) for all patients were 95, 21, and 94% for DVT, and 100, 26, and 100% for PE. This new D-dimer method demonstrates acceptable sensitivity in patients with PE and DVT and, based on the high NPV of this method, it can be used for the exclusion of thromboembolism.

KW - D-dimer

KW - Deep vein thrombosis

KW - Pulmonary embolism

UR - http://www.scopus.com/inward/record.url?scp=0034537963&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034537963&partnerID=8YFLogxK

U2 - 10.1097/00001721-200012000-00004

DO - 10.1097/00001721-200012000-00004

M3 - Article

C2 - 11132649

AN - SCOPUS:0034537963

VL - 11

SP - 715

EP - 721

JO - Blood Coagulation and Fibrinolysis

JF - Blood Coagulation and Fibrinolysis

SN - 0957-5235

IS - 8

ER -