A new method for measuring D-dimer using immunoturbidometry: A study of 255 patients with suspected pulmonary embolism and deep vein thrombosis

R. C. Gosselin, John T Owings, Garth H Utter, R. C. Jacoby, E. C. Larkin

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

D-Dimer testing has been suggested as a non-invasive method for the exclusion of pulmonary embolism (PE) and deep vein thrombosis (DVT). In this study, we compared a new method, the Miniquant D-dimer (Biopool International, Ventura, California, USA) to other previously validated D-dimer methods used for the purpose. Patients who were undergoing a definitive diagnostic study for thromboembolism had a blood sample drawn at that time. A whole-blood D-dimer (SimpliRed; Agen Biomedical Ltd, Brisbane, Australia) test was performed, and residual plasma was frozen and later analyzed using two enzyme-linked immunosorbent assay (ELISA) methods (D-dimer Gold; Agen, and Asserachrome D-Di; Stago International, Parsippany, New Jersey, USA) and the Miniquant D-dimer. Once all samples were analyzed, the correlation and accuracy of the Miniquant was compared with the ELISA method using Spearman's regression and Dunn's multiple paired comparison. All D-dimer methods were compared with radiographic studies. The data was analyzed collectively and segregated into in-patient (n = 112) and outpatient (n = 143) populations. The Miniquant D-dimer sensitivity, specificity and negative predictive value (NPV) for all patients were 95, 21, and 94% for DVT, and 100, 26, and 100% for PE. This new D-dimer method demonstrates acceptable sensitivity in patients with PE and DVT and, based on the high NPV of this method, it can be used for the exclusion of thromboembolism.

Original languageEnglish (US)
Pages (from-to)715-721
Number of pages7
JournalBlood Coagulation and Fibrinolysis
Volume11
Issue number8
DOIs
StatePublished - 2000

Keywords

  • D-dimer
  • Deep vein thrombosis
  • Pulmonary embolism

ASJC Scopus subject areas

  • Hematology

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