A new enzyme-linked immunosorbent assay system for human hepatic triglyceride lipase

Kazuya Miyashita, Junji Kobayashi, Shigeyuki Imamura, Noriaki Kinoshita, Kimber Stanhope, Peter J Havel, Katsuyuki Nakajima, Tetsuo Machida, Hiroyuki Sumino, Makoto Nara, Masami Murakami

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Background: The objective of this study was to establish a new sandwich based enzyme linked immunosorbent assay (ELISA) for measuring the protein mass of human hepatic triacylglyceride lipase (HTGL). Method: Two mouse monoclonal antibodies raised against human HTGL were used for the sandwich ELISA. The post-heparin plasma (PHP) samples obtained at a heparin dose of 50. unit/kg from 124 normolipidemic subjects were used for this ELISA. Results: The dynamic assay range of the developed ELISA for the HTGL was from 0.47 to 30. ng/ml. The CV was <. 7% in both intra- and inter-assays, and it did not cross-react with lipoprotein lipase or endothelial lipase (EL). The HTGL concentration in PHP showed a strong correlation with HTGL activity [n. =. 121, r. =. 0.778, p. <. 0.001]. There was a weak relation of HTGL concentration against high-density lipoprotein cholesterol (HDL-C) [n. =. 123, r. =. -. 0.229, p. =. 0.011] but no relations against total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), triglycerides (TG), small dense LDL, remnant like particles cholesterol (RLP-C) and RLP-TG were confirmed. Interestingly, a weak but positive correlation between HTGL concentration and EL concentration was shown [n. =. 122, p. =. 0.013, r. =. 0.224]. Conclusion: These results indicate that this new sandwich ELISA for measuring HTGL concentration in PHP can be applied in a daily clinical practice.

Original languageEnglish (US)
Pages (from-to)201-206
Number of pages6
JournalClinica Chimica Acta
Volume424
DOIs
StatePublished - Sep 23 2013

Keywords

  • ELISA
  • Endothelial lipase
  • Hepatic triglyceride lipase

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical

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    Miyashita, K., Kobayashi, J., Imamura, S., Kinoshita, N., Stanhope, K., Havel, P. J., Nakajima, K., Machida, T., Sumino, H., Nara, M., & Murakami, M. (2013). A new enzyme-linked immunosorbent assay system for human hepatic triglyceride lipase. Clinica Chimica Acta, 424, 201-206. https://doi.org/10.1016/j.cca.2013.06.016