A New Enzyme Immunoassay to Detect Antibodies to Arboviruses in the Blood of Wild Birds

R. E. Chiles, William Reisen

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

A new indirect enzyme immunoassay (EIA) was developed to screen wild bird sera for antibodies against western equine encephalomyelitis (WEE) and St. Louis encephalitis (SLE) viruses. The detector antibody was made by immunizing rabbits with serum proteins pooled from single species representatives of four bird orders and was conjugated with horseradish peroxidase to allow visualization with the ABTS substrate in an EIA plate reader set at 405 nm. The detector antibody recognized a wide range of bird species and was more accurate, sensitive, and specific than a hemaglutination inhibition test when compared to a plaque reduction neutralization test (PRNT). EIA positive sera frequently could not be confirmed by PRNT; however, practically all sera positive by PRNT also were positive by EIA. The new EIA has been incorporated into our field research program and has been used to economically screen over 10,000 wild bird sera from 124 species for antibodies against WEE and SLE.

Original languageEnglish (US)
Pages (from-to)123-135
Number of pages13
JournalJournal of Vector Ecology
Volume23
Issue number2
StatePublished - Dec 1 1998

Fingerprint

arboviruses
immunoassay
enzyme immunoassays
wild birds
antibody
serum
blood serum
blood
enzyme
bird
Western equine encephalomyelitis
neutralization tests
neutralization
antibodies
encephalitis
detectors
Saint Louis encephalitis virus
birds
research programs
research program

Keywords

  • Antibodies
  • Arboviruses
  • Birds
  • Enzyme immuno assay
  • SLE
  • WEE

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Ecology

Cite this

A New Enzyme Immunoassay to Detect Antibodies to Arboviruses in the Blood of Wild Birds. / Chiles, R. E.; Reisen, William.

In: Journal of Vector Ecology, Vol. 23, No. 2, 01.12.1998, p. 123-135.

Research output: Contribution to journalArticle

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