Abstract
A naturally occurring mutation was detected within the probe binding region targeting the envelope gene sequence of West Nile virus used in real-time polymerase chain reaction assays to test mosquito pools and other samples. A single C→T transition 6nt from the 5′ end of the 16mer in the envelope gene probe-binding region at genomic position 1,194 reduced assay sensitivity. The mutation first was detected in 2009 and persisted at a low prevalence into 2011. The mutation caused a 0.4% false negative error rate during 2011. These data emphasized the importance of confirmational testing and redundancy in surveillance systems relying on highly specific nucleic acid detection platforms.
Original language | English (US) |
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Pages (from-to) | 939-941 |
Number of pages | 3 |
Journal | Journal of Medical Entomology |
Volume | 49 |
Issue number | 4 |
DOIs | |
State | Published - Jul 1 2012 |
Keywords
- mosquito pool
- mutation
- probe binding region
- qRT-PCR
- surveillance
ASJC Scopus subject areas
- Insect Science
- veterinary(all)
- Infectious Diseases
- Parasitology