A naturally occurring mutation within the probe-binding region compromises a molecular-based west nile virus surveillance assay for mosquito pools (Diptera: Culicidae)

Aaron Brault, Ying Fang, Maureen Dannen, Michael Anishchenko, William Reisen

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

A naturally occurring mutation was detected within the probe binding region targeting the envelope gene sequence of West Nile virus used in real-time polymerase chain reaction assays to test mosquito pools and other samples. A single C→T transition 6nt from the 5′ end of the 16mer in the envelope gene probe-binding region at genomic position 1,194 reduced assay sensitivity. The mutation first was detected in 2009 and persisted at a low prevalence into 2011. The mutation caused a 0.4% false negative error rate during 2011. These data emphasized the importance of confirmational testing and redundancy in surveillance systems relying on highly specific nucleic acid detection platforms.

Original languageEnglish (US)
Pages (from-to)939-941
Number of pages3
JournalJournal of Medical Entomology
Volume49
Issue number4
DOIs
StatePublished - Jul 1 2012

Keywords

  • mosquito pool
  • mutation
  • probe binding region
  • qRT-PCR
  • surveillance

ASJC Scopus subject areas

  • Insect Science
  • veterinary(all)
  • Infectious Diseases
  • Parasitology

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