A nanoengineering approach for investigation and regulation of protein immobilization

Yih Horng Tan, Maozi Liu, Birte Nolting, Joan G. Go, Jacquelyn Gervay-Hague, Gang-yu Liu

Research output: Contribution to journalArticle

117 Citations (Scopus)

Abstract

It is known that protein attachment to surfaces depends sensitively upon the local structure and environment of the binding sites at the nanometer scale. Using nanografting and reversal nanografting, both atomic force microscopy (AFM)-based lithography techniques, protein binding sites with well-defined local environments are designed and engineered with nanometer precision. Three proteins, goat antibiotin immunoglobulin G (IgG), lysozyme, and rabbit immunoglobulin G, are immobilized onto these engineered surfaces. Strong dependence on the dimension and spatial distribution of protein binding sites are revealed in antibody recognition, covalent attachment via primary amine residues and surface-bound aldehyde groups. This investigation indicates that AFM-based nanolithography enables the production of protein nanostructures, and more importantly, protein-surface interactions at a molecular level can be regulated by changing the binding domains and their local environment at nanometer scale.

Original languageEnglish (US)
Pages (from-to)2374-2384
Number of pages11
JournalACS Nano
Volume2
Issue number11
DOIs
StatePublished - Nov 2008

Fingerprint

immobilization
Binding sites
Binding Sites
proteins
Proteins
Atomic force microscopy
Immunoglobulin G
Nanolithography
Muramidase
Aldehydes
Lithography
Spatial distribution
Amines
attachment
Nanostructures
Membrane Proteins
Antibodies
atomic force microscopy
goats
Enzymes

Keywords

  • Arrays of nanostructures
  • Atomic force microscopy
  • Local structures
  • Multipoint protein binding
  • Nanografting
  • Polyvalent interactions
  • Protein immobilization
  • Reversal nanografting
  • Self-assembled monolayers

ASJC Scopus subject areas

  • Engineering(all)
  • Materials Science(all)
  • Physics and Astronomy(all)

Cite this

A nanoengineering approach for investigation and regulation of protein immobilization. / Tan, Yih Horng; Liu, Maozi; Nolting, Birte; Go, Joan G.; Gervay-Hague, Jacquelyn; Liu, Gang-yu.

In: ACS Nano, Vol. 2, No. 11, 11.2008, p. 2374-2384.

Research output: Contribution to journalArticle

Tan, Yih Horng ; Liu, Maozi ; Nolting, Birte ; Go, Joan G. ; Gervay-Hague, Jacquelyn ; Liu, Gang-yu. / A nanoengineering approach for investigation and regulation of protein immobilization. In: ACS Nano. 2008 ; Vol. 2, No. 11. pp. 2374-2384.
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