A mutation burst during the acute phase of Helicobacter pylori infection in humans and rhesus macaques

Bodo Linz, Helen M. Windsor, John J. McGraw, Lori M. Hansen, John P. Gajewski, Lynn P. Tomsho, Caylie M. Hake, Jay V Solnick, Stephan C. Schuster, Barry J. Marshall

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The evolution rate and genetic changes that occur during chronic infection with Helicobacter pylori have been analysed, but little is known about the genomic changes during the initial, acute bacterial infection phase. Here we analyse the rate and pattern of genome evolution in H. pylori from the genomes of two input strains isolated from human volunteers with asymptomatic infection, and the genomes of two output strains collected 20 and 44 days after re-infection. Similarly, we analyse genome evolution in bacteria from the genome sequences of input and output strains sequentially taken after experimental infection of a rhesus macaque. The estimated mutation rate reveals a mutation burst during the acute infection phase that is over 10 times faster than the mutation rate during chronic infection, and orders of magnitude faster than mutation rates in any other bacteria. The elevated frequency of mutations in outer membrane protein genes suggests that the mutation burst facilitates rapid host adaptation of the bacteria.

Original languageEnglish (US)
Article number4165
JournalNature Communications
Volume5
DOIs
StatePublished - Jun 13 2014

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Helicobacter Infections
infectious diseases
mutations
Macaca mulatta
Helicobacter pylori
Mutation Rate
bursts
genome
Genes
Genome
Mutation
Infection
Bacteria
bacteria
Asymptomatic Infections
Molecular Evolution
Bacterial Infections
Volunteers
Membrane Proteins
output

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry(all)
  • Physics and Astronomy(all)

Cite this

Linz, B., Windsor, H. M., McGraw, J. J., Hansen, L. M., Gajewski, J. P., Tomsho, L. P., ... Marshall, B. J. (2014). A mutation burst during the acute phase of Helicobacter pylori infection in humans and rhesus macaques. Nature Communications, 5, [4165]. https://doi.org/10.1038/ncomms5165

A mutation burst during the acute phase of Helicobacter pylori infection in humans and rhesus macaques. / Linz, Bodo; Windsor, Helen M.; McGraw, John J.; Hansen, Lori M.; Gajewski, John P.; Tomsho, Lynn P.; Hake, Caylie M.; Solnick, Jay V; Schuster, Stephan C.; Marshall, Barry J.

In: Nature Communications, Vol. 5, 4165, 13.06.2014.

Research output: Contribution to journalArticle

Linz, B, Windsor, HM, McGraw, JJ, Hansen, LM, Gajewski, JP, Tomsho, LP, Hake, CM, Solnick, JV, Schuster, SC & Marshall, BJ 2014, 'A mutation burst during the acute phase of Helicobacter pylori infection in humans and rhesus macaques', Nature Communications, vol. 5, 4165. https://doi.org/10.1038/ncomms5165
Linz, Bodo ; Windsor, Helen M. ; McGraw, John J. ; Hansen, Lori M. ; Gajewski, John P. ; Tomsho, Lynn P. ; Hake, Caylie M. ; Solnick, Jay V ; Schuster, Stephan C. ; Marshall, Barry J. / A mutation burst during the acute phase of Helicobacter pylori infection in humans and rhesus macaques. In: Nature Communications. 2014 ; Vol. 5.
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