A multimode optical imaging system for preclinical applications in Vivo: Technology development, multiscale imaging, and chemotherapy assessment

Jae Youn Hwang, Sebastian Wachsmann-Hogiu, V. Krishnan Ramanujan, Julia Ljubimova, Zeev Gross, Harry B. Gray, Lali K. Medina-Kauwe, Daniel L. Farkas

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Purpose: Several established optical imaging approaches have been applied, usually in isolation, to preclinical studies; however, truly useful in vivo imaging may require a simultaneous combination of imaging modalities to examine dynamic characteristics of cells and tissues. We developed a new multimode optical imaging system designed to be application-versatile, yielding high sensitivity, and specificity molecular imaging. Procedures: We integrated several optical imaging technologies, including fluorescence intensity, spectral, lifetime, intravital confocal, two-photon excitation, and bioluminescence, into a single system that enables functional multiscale imaging in animal models. Results: The approach offers a comprehensive imaging platform for kinetic, quantitative, and environmental analysis of highly relevant information, with micro-to-macroscopic resolution. Applied to small animals in vivo, this provides superior monitoring of processes of interest, represented here by chemo-/nanoconstruct therapy assessment. Conclusions: This new system is versatile and can be optimized for various applications, of which cancer detection and targeted treatment are emphasized here.

Original languageEnglish (US)
Pages (from-to)431-442
Number of pages12
JournalMolecular Imaging and Biology
Volume14
Issue number4
DOIs
StatePublished - Aug 2012

Keywords

  • Chemotherapy
  • Corroles
  • Fluorescence lifetime
  • In vivo
  • Multimode
  • Nanoconstruct
  • Preclinical
  • Spectral analysis
  • Wide-field two-photon excitation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Radiology Nuclear Medicine and imaging

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