A mouse cytoplasmic exoribonuclease (mXRN1p) with preference for G4 tetraplex substrates

Vladimir I. Bashkirov, Harry Scherthan, Jachen A. Solinger, Jean Marie Buerstedde, Wolf Dietrich Heyer

Research output: Contribution to journalArticlepeer-review

273 Scopus citations


Exoribonucleases are important enzymes for the turnover of cellular RNA species. We have isolated the first mammalian cDNA from mouse demonstrated to encode a 5'-3' exoribonuclease. The structural conservation of the predicted protein and complementation data in Saccharomyces cerevisiae suggest a role in cytoplasmic mRNA turnover and pre-rRNA processing similar to that of the major cytoplasmic exoribonuclease Xrn1p in yeast. Therefore, a key component of the mRNA decay system in S. cerevisiae has been conserved in evolution from yeasts to mammals. The purified mouse protein (mXRN1p) exhibited a novel substrate preference for G4 RNA tetraplex-containing substrates demonstrated in binding and hydrolysis experiments. mXRN1p is the first RNA turnover function that has been localized in the cytoplasm of mammalian cells. mXRN1p was distributed in small granules and was highly enriched in discrete, prominent foci. The specificity of mXRN1p suggests that RNAs containing G4 tetraplex structures may occur in vivo and may have a role in RNA turnover.

Original languageEnglish (US)
Pages (from-to)761-773
Number of pages13
JournalJournal of Cell Biology
Issue number4
StatePublished - 1997
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology


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