A more valid measurement of low-density lipoprotein cholesterol in diabetic patients

Shaina Hirany, Dai Li, Ishwarlal Jialal

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

PURPOSE: This study was conducted to determine if the direct low- density lipoprotein (LDL) cholesterol assay would provide a more valid measure of LDL cholesterol in diabetic patients compared with the Friedewald equation. PATIENTS AND METHODS: Fasting plasma from 148 diabetic patients, 40 with insulin-dependent diabetes (IDDM) and 108 with non-insulin-dependent diabetes (NIDDM) with triglyceride levels <400 mg/dL, were analyzed for LDL cholesterol using the Friedewald equation, the direct LDL assay, and beta- quantification. Forty-six diabetic patients with triglyceride levels ≤400 mg/dL were also studied to determine the validity of the direct LDL cholesterol assay with hypertriglyceridemia. RESULTS: The Friedewald equation and the direct LDL cholesterol assay correlated well with beta-quantification (r = 0.969 and r = 0.971, respectively) for LDL cholesterol determination in diabetic patients. Although the Friedewald equation in comparison with beta- quantification underestimated (8%) LDL cholesterol values in diabetic patients, the direct LDL cholesterol assay had a mean bias of <1%. Also, the underestimation by the Friedewald equation exceeded 10% for the triglyceride subgroup of 200 to 400 mg/dL. Furthermore, the accuracy of the direct LDL cholesterol assay was superior to the Friedewald equation since LDL cholesterol levels determined by the two methods coincided within ± 10% of beta-quantification in 85% and 68% of diabetic patients, respectively (P = 0.0005). Similar results for both the Friedewald equation and the direct LDL cholesterol assay in comparison with beta-quantification were seen when diabetic patients were subgrouped into IDDM and NIDDM. Also, the direct LDL cholesterol assay appeared to provide a reliable estimate in patients with triglycerides ≤400 mg/dL. CONCLUSION: The results of our studies indicate that the direct LDL cholesterol assay is a more reliable and accurate method than the Friedewaid formula for LDL cholesterol determination in diabetic patients and is more rapid and cost effective than the reference method.

Original languageEnglish (US)
Pages (from-to)48-53
Number of pages6
JournalAmerican Journal of Medicine
Volume102
Issue number1
DOIs
StatePublished - Jan 1997
Externally publishedYes

ASJC Scopus subject areas

  • Nursing(all)

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