Abstract
A monoclonal antibody to the surface membrane of rat Schwann cells was generated. This antibody bound to Schwann cell surfaces but not to fibroblasts in mixed neonatal sciatic nerve cultures. It did not bind to cells in newborn rat brain or corpus callosum cultures that contained oligodendrocytes, astrocytes, fibroblasts and neurons. The target antigen for this monoclonal antibody disappeared from Schwann cell surface after 5 days in culture. At this time, addition of 10-3 M 8-bromo cyclic AMP or 10-3 M dibutyryl cyclic AMP resulted in the reappearance within 2 days, of the target antigen on Schwann cell surface membrane. The reappearance of this epitope parallels that of surface galactocerebroside on Schwann cells when incubated with cAMP analogues. Re-expression of the target antigen for this monoclonal antibody in the presence of cAMP is evidence that cAMP may be a signal for synthesis of a variety of myelin molecules and as such could be a signal for myelination of axons by Schwann cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 205-211 |
| Number of pages | 7 |
| Journal | Brain Research |
| Volume | 425 |
| Issue number | 2 |
| DOIs | |
| State | Published - Nov 10 1987 |
| Externally published | Yes |
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Keywords
- Cyclic AMP
- Monoclonal antibody
- Myelination
- Schwann cell
- Surface membrane molecule
ASJC Scopus subject areas
- Developmental Biology
- Molecular Biology
- Clinical Neurology
- Neuroscience(all)
Cite this
A monoclonal antibody to Schwann cell surface membrane recognizes a cAMP inducible epitope. / Rostami, Abdolmohammad; Sobue, Gen; Lisak, Robert P.; Pleasure, David E.
In: Brain Research, Vol. 425, No. 2, 10.11.1987, p. 205-211.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A monoclonal antibody to Schwann cell surface membrane recognizes a cAMP inducible epitope
AU - Rostami, Abdolmohammad
AU - Sobue, Gen
AU - Lisak, Robert P.
AU - Pleasure, David E
PY - 1987/11/10
Y1 - 1987/11/10
N2 - A monoclonal antibody to the surface membrane of rat Schwann cells was generated. This antibody bound to Schwann cell surfaces but not to fibroblasts in mixed neonatal sciatic nerve cultures. It did not bind to cells in newborn rat brain or corpus callosum cultures that contained oligodendrocytes, astrocytes, fibroblasts and neurons. The target antigen for this monoclonal antibody disappeared from Schwann cell surface after 5 days in culture. At this time, addition of 10-3 M 8-bromo cyclic AMP or 10-3 M dibutyryl cyclic AMP resulted in the reappearance within 2 days, of the target antigen on Schwann cell surface membrane. The reappearance of this epitope parallels that of surface galactocerebroside on Schwann cells when incubated with cAMP analogues. Re-expression of the target antigen for this monoclonal antibody in the presence of cAMP is evidence that cAMP may be a signal for synthesis of a variety of myelin molecules and as such could be a signal for myelination of axons by Schwann cells.
AB - A monoclonal antibody to the surface membrane of rat Schwann cells was generated. This antibody bound to Schwann cell surfaces but not to fibroblasts in mixed neonatal sciatic nerve cultures. It did not bind to cells in newborn rat brain or corpus callosum cultures that contained oligodendrocytes, astrocytes, fibroblasts and neurons. The target antigen for this monoclonal antibody disappeared from Schwann cell surface after 5 days in culture. At this time, addition of 10-3 M 8-bromo cyclic AMP or 10-3 M dibutyryl cyclic AMP resulted in the reappearance within 2 days, of the target antigen on Schwann cell surface membrane. The reappearance of this epitope parallels that of surface galactocerebroside on Schwann cells when incubated with cAMP analogues. Re-expression of the target antigen for this monoclonal antibody in the presence of cAMP is evidence that cAMP may be a signal for synthesis of a variety of myelin molecules and as such could be a signal for myelination of axons by Schwann cells.
KW - Cyclic AMP
KW - Monoclonal antibody
KW - Myelination
KW - Schwann cell
KW - Surface membrane molecule
UR - http://www.scopus.com/inward/record.url?scp=0023588139&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023588139&partnerID=8YFLogxK
U2 - 10.1016/0006-8993(87)90502-6
DO - 10.1016/0006-8993(87)90502-6
M3 - Article
C2 - 2448004
AN - SCOPUS:0023588139
VL - 425
SP - 205
EP - 211
JO - Brain Research
JF - Brain Research
SN - 0006-8993
IS - 2
ER -