A modular lymphographic magnetic resonance imaging contrast agent

Contrast enhancement with DNA transfection potential

Erik R Wisner, Kimberly L. Aho-Sharon, Michael J. Bennett, Sharron G. Penn, Carlito B Lebrilla, Michael H. Nantz

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A gadolinium-chelated liposomal contrast agent has been prepared, and magnetic resonance imaging (MRI) efficacy has been examined by indirect magnetic resonance lymphography. A lipidic N,N'-dimethylethylenediamine derivative (4) containing a 10,12-diyne-diacyl domain was treated with DTPA anhydride followed by GdCl3 complexation. The complex was confirmed using MALDI spectrometry. An equimolar mixture of the Gd-chelate lipid and a commercially available diyne-PE was formulated as a liposome suspension and irradiated with UV light prior to imaging experiments. Subcutaneous injection of the liposomal gadolinium agent and subsequent MRI of rabbit axillary and popliteal lymph nodes revealed significant contrast enhancement up to 4 h postinjection. To explore the possibility of imaging a DNA transfection event, the gadolinium contrast mixture was formulated with the cationic transfection lipid DOTAP and complexed with the reporter gene encoding luciferase. DNA transfection studies on the NIH3T3 cell line confirmed the transfection activity of the dual-purpose contrast agent and exemplified the potential toward development of an imaging and DNA delivery vehicle.

Original languageEnglish (US)
Pages (from-to)3992-3996
Number of pages5
JournalJournal of Medicinal Chemistry
Volume40
Issue number25
DOIs
StatePublished - 1997

Fingerprint

Magnetic resonance
Contrast Media
Transfection
Gadolinium
Magnetic Resonance Imaging
Imaging techniques
DNA
Lipids
Lymphography
Pentetic Acid
Anhydrides
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Subcutaneous Injections
Ultraviolet Rays
Luciferases
Reporter Genes
Gene encoding
Liposomes
Spectrum Analysis
Suspensions

ASJC Scopus subject areas

  • Organic Chemistry

Cite this

A modular lymphographic magnetic resonance imaging contrast agent : Contrast enhancement with DNA transfection potential. / Wisner, Erik R; Aho-Sharon, Kimberly L.; Bennett, Michael J.; Penn, Sharron G.; Lebrilla, Carlito B; Nantz, Michael H.

In: Journal of Medicinal Chemistry, Vol. 40, No. 25, 1997, p. 3992-3996.

Research output: Contribution to journalArticle

Wisner, Erik R ; Aho-Sharon, Kimberly L. ; Bennett, Michael J. ; Penn, Sharron G. ; Lebrilla, Carlito B ; Nantz, Michael H. / A modular lymphographic magnetic resonance imaging contrast agent : Contrast enhancement with DNA transfection potential. In: Journal of Medicinal Chemistry. 1997 ; Vol. 40, No. 25. pp. 3992-3996.
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