A mitotic kinesin-6, Pav-KLP, mediates interdependent cortical reorganization and spindle dynamics in Drosophilaembryos

Patrizia Sommi, Revathi Ananthakrishnan, Dhanya K. Cheerambathur, Mijung Kwon, Sandra Morales-Mulia, Ingrid Brust-Mascher, Alex Mogilner

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

We investigated the role of Pav-KLP, a kinesin-6, in the coordination of spindle and cortical dynamics during mitosis in Drosophila embryos. In vitro, Pav-KLP behaves as a dimer. In vivo, it localizes to mitotic spindles and furrows. Inhibition of Pav-KLP causes defects in both spindle dynamics and furrow ingression, as well as causing changes in the distribution of actin and vesicles. Thus, Pav-KLP stabilizes the spindle by crosslinking interpolar microtubule bundles and contributes to actin furrow formation possibly by transporting membrane vesicles, actin and/or actin regulatory molecules along astral microtubules. Modeling suggests that furrow ingression during cellularization depends on: (1) a Pav-KLP-dependent force driving an initial slow stage of ingression; and (2) the subsequent Pav-KLP-driven transport of actin- and membrane-containing vesicles to the furrow during a fast stage of ingression. We hypothesize that Pav-KLP is a multifunctional mitotic motor that contributes both to bundling of interpolar microtubules, thus stabilizing the spindle, and to a biphasic mechanism of furrow ingression by pulling down the furrow and transporting vesicles that deliver new material to the descending furrow.

Original languageEnglish (US)
Pages (from-to)1862-1872
Number of pages11
JournalJournal of Cell Science
Volume123
Issue number11
DOIs
StatePublished - Jun 1 2010

Keywords

  • Actin
  • Kinesin-6
  • Mitosis
  • Nuf
  • Pavarotti
  • Vesicles

ASJC Scopus subject areas

  • Cell Biology
  • Medicine(all)

Fingerprint Dive into the research topics of 'A mitotic kinesin-6, Pav-KLP, mediates interdependent cortical reorganization and spindle dynamics in Drosophilaembryos'. Together they form a unique fingerprint.

  • Cite this

    Sommi, P., Ananthakrishnan, R., Cheerambathur, D. K., Kwon, M., Morales-Mulia, S., Brust-Mascher, I., & Mogilner, A. (2010). A mitotic kinesin-6, Pav-KLP, mediates interdependent cortical reorganization and spindle dynamics in Drosophilaembryos. Journal of Cell Science, 123(11), 1862-1872. https://doi.org/10.1242/jcs.064048