A mitotic kinesin-6, Pav-KLP, mediates interdependent cortical reorganization and spindle dynamics in Drosophilaembryos

Patrizia Sommi, Revathi Ananthakrishnan, Dhanya K. Cheerambathur, Mijung Kwon, Sandra Morales-Mulia, Ingrid Brust-Mascher, Alex Mogilner

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We investigated the role of Pav-KLP, a kinesin-6, in the coordination of spindle and cortical dynamics during mitosis in Drosophila embryos. In vitro, Pav-KLP behaves as a dimer. In vivo, it localizes to mitotic spindles and furrows. Inhibition of Pav-KLP causes defects in both spindle dynamics and furrow ingression, as well as causing changes in the distribution of actin and vesicles. Thus, Pav-KLP stabilizes the spindle by crosslinking interpolar microtubule bundles and contributes to actin furrow formation possibly by transporting membrane vesicles, actin and/or actin regulatory molecules along astral microtubules. Modeling suggests that furrow ingression during cellularization depends on: (1) a Pav-KLP-dependent force driving an initial slow stage of ingression; and (2) the subsequent Pav-KLP-driven transport of actin- and membrane-containing vesicles to the furrow during a fast stage of ingression. We hypothesize that Pav-KLP is a multifunctional mitotic motor that contributes both to bundling of interpolar microtubules, thus stabilizing the spindle, and to a biphasic mechanism of furrow ingression by pulling down the furrow and transporting vesicles that deliver new material to the descending furrow.

Original languageEnglish (US)
Pages (from-to)1862-1872
Number of pages11
JournalJournal of Cell Science
Volume123
Issue number11
DOIs
StatePublished - Jun 1 2010

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Kinesin
Actins
Microtubules
Spindle Apparatus
Membranes
Mitosis
Drosophila
Embryonic Structures

Keywords

  • Actin
  • Kinesin-6
  • Mitosis
  • Nuf
  • Pavarotti
  • Vesicles

ASJC Scopus subject areas

  • Cell Biology
  • Medicine(all)

Cite this

Sommi, P., Ananthakrishnan, R., Cheerambathur, D. K., Kwon, M., Morales-Mulia, S., Brust-Mascher, I., & Mogilner, A. (2010). A mitotic kinesin-6, Pav-KLP, mediates interdependent cortical reorganization and spindle dynamics in Drosophilaembryos. Journal of Cell Science, 123(11), 1862-1872. https://doi.org/10.1242/jcs.064048

A mitotic kinesin-6, Pav-KLP, mediates interdependent cortical reorganization and spindle dynamics in Drosophilaembryos. / Sommi, Patrizia; Ananthakrishnan, Revathi; Cheerambathur, Dhanya K.; Kwon, Mijung; Morales-Mulia, Sandra; Brust-Mascher, Ingrid; Mogilner, Alex.

In: Journal of Cell Science, Vol. 123, No. 11, 01.06.2010, p. 1862-1872.

Research output: Contribution to journalArticle

Sommi, P, Ananthakrishnan, R, Cheerambathur, DK, Kwon, M, Morales-Mulia, S, Brust-Mascher, I & Mogilner, A 2010, 'A mitotic kinesin-6, Pav-KLP, mediates interdependent cortical reorganization and spindle dynamics in Drosophilaembryos', Journal of Cell Science, vol. 123, no. 11, pp. 1862-1872. https://doi.org/10.1242/jcs.064048
Sommi P, Ananthakrishnan R, Cheerambathur DK, Kwon M, Morales-Mulia S, Brust-Mascher I et al. A mitotic kinesin-6, Pav-KLP, mediates interdependent cortical reorganization and spindle dynamics in Drosophilaembryos. Journal of Cell Science. 2010 Jun 1;123(11):1862-1872. https://doi.org/10.1242/jcs.064048
Sommi, Patrizia ; Ananthakrishnan, Revathi ; Cheerambathur, Dhanya K. ; Kwon, Mijung ; Morales-Mulia, Sandra ; Brust-Mascher, Ingrid ; Mogilner, Alex. / A mitotic kinesin-6, Pav-KLP, mediates interdependent cortical reorganization and spindle dynamics in Drosophilaembryos. In: Journal of Cell Science. 2010 ; Vol. 123, No. 11. pp. 1862-1872.
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