A mitochondrial protease with two catalytic subunits of nonoverlapping specificities

Jodi Nunnari, Thomas D. Fox, Peter Walter

Research output: Contribution to journalArticle

191 Citations (Scopus)

Abstract

The mitochondrial inner membrane protease is required for the maturation of mitochondrial proteins that are delivered to the intermembrane space. In the yeast Saccharomyces cerevisiae, this protease is now shown to be a complex that contains two catalytic subunits, Imp2p and the previously identified Imp1p. Primary structure similarity indicates that Imp1p and Imp2p are related to each other and to the family of eubacterial and eukaryotic signal peptidases. Imp1p and Imp2p have separate, nonoverlapping substrate specificities. In addition to its catalyzing the cleavage of intermembrane space sorting signals, Imp2p is required for the stable and functional expression of Imp1p. Thus, inner membrane protease, and by analogy eukaryotic multisubunit signal peptidases, may have acquired multiple catalytic subunits by gene duplication to broaden their range of substrate specificity.

Original languageEnglish (US)
Pages (from-to)1997-2004
Number of pages8
JournalScience
Volume262
Issue number5142
StatePublished - Dec 24 1993
Externally publishedYes

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Catalytic Domain
Peptide Hydrolases
Substrate Specificity
Gene Duplication
Mitochondrial Proteins
Mitochondrial Membranes
Saccharomyces cerevisiae
Yeasts
Membranes
type I signal peptidase

ASJC Scopus subject areas

  • General

Cite this

Nunnari, J., Fox, T. D., & Walter, P. (1993). A mitochondrial protease with two catalytic subunits of nonoverlapping specificities. Science, 262(5142), 1997-2004.

A mitochondrial protease with two catalytic subunits of nonoverlapping specificities. / Nunnari, Jodi; Fox, Thomas D.; Walter, Peter.

In: Science, Vol. 262, No. 5142, 24.12.1993, p. 1997-2004.

Research output: Contribution to journalArticle

Nunnari, J, Fox, TD & Walter, P 1993, 'A mitochondrial protease with two catalytic subunits of nonoverlapping specificities', Science, vol. 262, no. 5142, pp. 1997-2004.
Nunnari J, Fox TD, Walter P. A mitochondrial protease with two catalytic subunits of nonoverlapping specificities. Science. 1993 Dec 24;262(5142):1997-2004.
Nunnari, Jodi ; Fox, Thomas D. ; Walter, Peter. / A mitochondrial protease with two catalytic subunits of nonoverlapping specificities. In: Science. 1993 ; Vol. 262, No. 5142. pp. 1997-2004.
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