A mitochondrial-focused genetic interaction map reveals a scaffold-like complex required for inner membrane organization in mitochondria

Suzanne Hoppins, Sean Collins, Ann Cassidy-Stone, Eric Hummel, Rachel M. DeVay, Laura L. Lackner, Benedikt Westermann, Maya Schuldiner, Jonathan S. Weissman, Jodi Nunnari

Research output: Contribution to journalArticle

283 Scopus citations

Abstract

To broadly explore mitochondrial structure and function as well as the communication of mitochondria with other cellular pathways, we constructed a quantitative, high-density genetic interaction map (the MITO-MAP) in Saccharomyces cerevisiae. The MITO-MAP provides a comprehensive view of mitochondrial function including insights into the activity of uncharacterized mitochondrial proteins and the functional connection between mitochondria and the ER. The MITO-MAP also reveals a large inner membrane-associated complex, which we term MitOS for mitochondrial organizing structure, comprised of Fcj1/Mitofilin, a conserved inner membrane protein, and five additional components. MitOS physically and functionally interacts with both outer and inner membrane components and localizes to extended structures that wrap around the inner membrane. We show that MitOS acts in concert with ATP synthase dimers to organize the inner membrane and promote normal mitochondrial morphology. We propose that MitOS acts as a conserved mitochondrial skeletal structure that differentiates regions of the inner membrane to establish the normal internal architecture of mitochondria.

Original languageEnglish (US)
Pages (from-to)323-340
Number of pages18
JournalJournal of Cell Biology
Volume195
Issue number2
DOIs
StatePublished - Oct 17 2011

ASJC Scopus subject areas

  • Cell Biology

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    Hoppins, S., Collins, S., Cassidy-Stone, A., Hummel, E., DeVay, R. M., Lackner, L. L., Westermann, B., Schuldiner, M., Weissman, J. S., & Nunnari, J. (2011). A mitochondrial-focused genetic interaction map reveals a scaffold-like complex required for inner membrane organization in mitochondria. Journal of Cell Biology, 195(2), 323-340. https://doi.org/10.1083/jcb.201107053