A missense mutation in damage-specific DNA binding protein 2 is a genetic risk factor for limbal squamous cell carcinoma in horses

Rebecca Bellone, Jiayin Liu, Jessica L. Petersen, Maura Mack, Moriel Singer-Berk, Cord Drögemüller, Julia Malvick, Barbara Wallner, Gottfried Brem, Cecilia Penedo, Mary Utter

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Squamous cell carcinoma (SCC) is the most common cancer of the equine eye, frequently originating at the limbus, with the potential to invade the cornea, cause visual impairment, and result in loss of the eye. Several breeds of horses have a high occurrence of limbal SCC implicating a genetic basis for limbal SCC predisposition. Pedigree analysis in the Haflinger breed supports a simple recessive mode of inheritance and a genome-wide association study (N = 23) identified a 1.5 Mb locus on ECA12 significantly associated with limbal SCC (Pcorrected= 0.04). Sequencing the most physiologically relevant gene from this locus, damage specific DNA binding protein 2 (DDB2), identified a missense mutation (c.1013 C > T p.Thr338Met) that was strongly associated with limbal SCC (P = 3.41 × 10−10). Genotyping 42 polymorphisms narrowed the ECA12 candidate interval to 483 kb but did not identify another variant that was more strongly associated. DDB2 binds to ultraviolet light damaged DNA and recruits other proteins to perform global genome nucleotide excision repair. Computational modeling predicts this mutation to be deleterious by altering conformation of the β loop involved in photolesion recognition. This DDB2 variant was also detected in two other closely related breeds with reported cases of ocular SCC, the Belgian and the Percheron, suggesting it may also be a SCC risk factor in these breeds. Furthermore, in humans xeroderma pigmentosum complementation group E, a disease characterized by sun sensitivity and increased risk of cutaneous SCC and melanomas, is explained by mutations in DDB2. Cross-species comparison remains to be further evaluated.

Original languageEnglish (US)
Pages (from-to)342-353
Number of pages12
JournalInternational Journal of Cancer
Volume141
Issue number2
DOIs
StatePublished - Jul 15 2017

Fingerprint

DNA-Binding Proteins
Missense Mutation
Horses
Squamous Cell Carcinoma
Eye Neoplasms
Mutation
Genome-Wide Association Study
Vision Disorders
Solar System
Pedigree
Ultraviolet Rays
DNA Repair
Cornea
Melanoma
Genome
Skin
DNA
Genes

Keywords

  • damage-specific binding protein 2
  • genetics
  • horse
  • ocular cancer

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

A missense mutation in damage-specific DNA binding protein 2 is a genetic risk factor for limbal squamous cell carcinoma in horses. / Bellone, Rebecca; Liu, Jiayin; Petersen, Jessica L.; Mack, Maura; Singer-Berk, Moriel; Drögemüller, Cord; Malvick, Julia; Wallner, Barbara; Brem, Gottfried; Penedo, Cecilia; Utter, Mary.

In: International Journal of Cancer, Vol. 141, No. 2, 15.07.2017, p. 342-353.

Research output: Contribution to journalArticle

Bellone, Rebecca ; Liu, Jiayin ; Petersen, Jessica L. ; Mack, Maura ; Singer-Berk, Moriel ; Drögemüller, Cord ; Malvick, Julia ; Wallner, Barbara ; Brem, Gottfried ; Penedo, Cecilia ; Utter, Mary. / A missense mutation in damage-specific DNA binding protein 2 is a genetic risk factor for limbal squamous cell carcinoma in horses. In: International Journal of Cancer. 2017 ; Vol. 141, No. 2. pp. 342-353.
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