A mini-STR typing system for degraded equine DNA

T. J. Kun, E. J. Wictum, Cecilia Penedo

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Degraded biological samples are a challenge for testing laboratories. Genotyping success can be improved through the use of mini-STRs, by which primers are placed adjacent to the repeat motifs to reduce amplicon size. Here, we present a genetic profiling system comprising 13 autosomal and one X-linked dinucleotide-repeat markers and the SRY gene based on the internationally accepted equine parentage panel. The markers are divided into two panels with all alleles falling at or below 182 bp. The application of this method significantly increases the ability to profile difficult samples and to provide discriminating results to clients.

Original languageEnglish (US)
Pages (from-to)464-466
Number of pages3
JournalAnimal Genetics
Volume49
Issue number5
DOIs
StatePublished - Oct 1 2018

Keywords

  • degraded DNA
  • equine STR
  • Equus caballus
  • horse
  • urine

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics

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